Generating Standard Curve and Determining Concentration of Unknown Sample in Excel

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In this video, you will learn how to Generate a Standard Curve and determine Unknown Concentrations in Excel by a Simple Method. A standard curve is used to accurately determine the concentration of your sample from the signal generated by an assay. I will show you how to create a standard linear curve using Microsoft Excel and how to use it to calculate unknown sample values. I will use imaginary data of concentration and absorbance as an example. I have used excel 2016 to generate a standard curve and determine an unknown concentration of compound. You can also use other versions of excel such as excel 2013, excel 2019 and excel 365.

#standardcurve #teachingjunction #excel #concentrationvsabsorbance

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where did you get that 0.484, answer please

aishakhalfan
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can we use this equation to calculate conc. if unknown sample absorbance value higher than ploted values?

nausheenghaffar
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Can you make a video on how to compare our results with a standard?

nailabashir-gb
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How did you find the value of concentration, as well as absorbance? please let me know

Thank you

asifmohammed
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how do we know that the calibrated graphic that we made is correct sir?

_aufa
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Why u use 0.0249x like where did u get the value?

nurainshamilabintikamalari
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Please find the unknown concentration without the trend line equation. It's highly advicable not use the equation in most experimental reports

thebluebeyond
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you thumbnails videos hidden the video at the end of the video
please remove it

oursoils
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Don't use English title for your video if you are not making an english video. It is very cheep when you do a video in your own language and use a title from another languages.

Urarto