Explain the Calibration Curve method & Standard addition method | Spectroscopy | Analytical

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The concentration of unknown in flame photometry is determined by two methods:

(i) Calibration method: We take some known concentration of the given soln and find the intensity of the emitted light. A graph of intensity measured Vs Conc is plotted. Now the intensity of the test solution is measured. Take this value on the intensity axis and draw the line parallel to the X-axis where it cuts the standard curve, from there draw the perpendicular to the conc axis which will give the concentration.

(ii) Standard addition method: In this method the intensity of the light of the test solution is measured with reference to the blank. Now known increasing amount of the element to be determined are then added to a number of the test solutions and the solutions are diluted to the same volume in each case. Intensity of the light of each solution is determined. A graph of readings Vs slandered conc added is plotted the graph will be straight line this line is extrapolated on conc axis which gives the concentration.

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U saved my life I'm going to have my test tomorrow and this thing was confusing me alot thanku so much

anmolyousaf
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Even though you are quite hard to understand this is the first video which explained it to me successfully!

Freizeitflugsphaere
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Thank you so much!

this is by far the simplest and most effective explanation!!!!

rileymurtagh
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Excellent educative video, Saludos desde México!

elielromero
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Even though there are still some lingering questions in mind. It is simply a superb explanation

nayudugaya
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Help with a question A compound absorb light in 227 nm. The standard curve calibration for the compound is Abs = 372x + 0, 6432 which the concentration is g/100mL. The technique was analytical standard adding from fluoxetine hydrochloride (C17H18F3NO.HCl - molar mass = 345, 79 g/mol). So determine the concentration in mol/L from the analysed solution. Show all calcs progression.

fabioverdi
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nice, how the extrapolated graph is saying the concentration?kindly explain

edubrain
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concentration of analyte before any analyte is added. I UNDERSTAND BUT
PLS SIR EXPLAIN HOW ITS CONCENTRATION IS IN MINUS IN SECOND METHOD ?
REPLY SIR....

dhruvsonani
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Can you explain how to get extrapolation?

amaliahmutmainnah
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Excuse me, what does the 'minus concentration' mean?

TheAlien
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What happens when I have 4 intensities for the blank? wich value should I plot in the graph for the blank?

lizfigueredo
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Background music is like ..im watching alladin.

jyotivishwakarma