How To Interpolate A Standard Curve In GraphPad Prism

In this video tutorial, I will show you how to interpolate a standard curve by using GraphPad Prism.

In the example, I have performed an ELISA experiment where I need to calculate the concentrations of my samples with known absorbance values, through the use of a standard curve. The standard curve in this example is that of a hyperbola relationship.

GraphPad Prism version used: 6

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[mlgc]

This saved my life and my Masters's thesis work. Thank you so much!

[claudiacarrillo]

thank you sm, you just saved my life and career

[sanjuktachakraborty]

Thank you for the tutorial. Helped me a lot.

[peacefulcali]

Thank you for the video. Can you get LLOQ and ULOQ data from this as well?

[fishflavoredicecream]

Hi, may I ask, how does this take into account the dilution of serum samples? For example, what if on a single plate with two treatment groups and a standard, one treatment group sera were diluted as 1:500 and the other as 1:200? Can they still use the same standard?

[mikenaughton]

What about the curve performance? How do you find out or display the concentration of each calibration point? If you were running duplicates I'd want to see the %RE (accuracy) to the spiked conc and the %CV (precision) of replicates for each level. Then the acceptability of the analytical run can be determined (assuming 20 - 30% acceptance criteria).

[prajithnambiar]

hi how to interpolate in graphpad prism 5

[dr.asaadaladlaan]

IT IS NOT ACCURATE, TRY PUT THE SAME ABSORBANCE OF THE S1 OR S2 AS UNKNOWN. YOU WILL SEE DIFFERENT CONCENTRATION. LET SAY THAT S1 (30 NG/ML) WITH OD (0.667) WHEN YOU PUT THE SAME OD 0.667 AS AN UNKNOWN THAT WILL GIVE YOU CONCENTRATION 32.45 AND NOT 30. IT SHOULD BE 30 NG/ML SINCE THE OD IS THE SAME 0.667. THAT RESULT MIGHT SHIFT RESULTS TO POSITIVE INSTEAD OF NEGATIVE.