How to do a hematology stain (Wright-Giemsa stain) Laboratory Tutorial Procedure

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Wright-Giemsa Stains, also known as Romanowsky Stains, are stains used in hematology and cytological studies to differentiate cells in microscopic examinations of peripheral blood and bone marrow specimens.

These stains can also be applied to detect the presence of parasites in the blood when rapid results are needed, such as for the detection of the Plasmodium parasite that causes malaria.

There are a variety of Romanowsky stain types that apply the same principle. They include:
1. Giemsa stain
2. Wright and Wright-Giemsa stain
3. May-Grunwald stain
4. Leishman stain

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00:00 Intro
00:09 History of Wright-Giemsa stains
02:49 Preparing specimen smears
03:12 Basic blood slide preparation
04:48 Prepare reagents for the Wright-Giemsa stain
06:18 Performing the Wright-Giemsa stain
06:40 Step 1 Fix slide with methanol
07:06 Step 2 Place slide in Wright-Giemsa Stain
07:29 Step 3 Remove and drain slide
07:39 Step 4 Place slide in buffer
07:57 Step 5 Remove and drain slide again
08:09 Step 6 Place slide in rinse solution
08:34 Step 7 Dry slide
08:44 Step 8 View slide under microscope
09:04 Table guide
09:20 Ordering info
09:46 Hardy Diagnostics Close

Filmed and Edited by Brian Tom
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Really good video. Needs more traction and views. Underrated

AthanaSus
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Question? At 6 minutes you state Rapid Stat Stain requires no buffer and can skip to the rinse step but then at 7:50 it suggests dipping the slide in buffer solution (?) This video over all is fantastic for explaining the smear staining process but I want to use Stat Stain with less fuss about preparing slide as I'd be doing this at home at the kitchen table with a microscope looking for changes in Eosinophil counts. Could you please clarify for me the difference between 6 minutes and at 7mins 30 seconds? Thanks!

josephmaloney-jnbg
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Clear, concise & helpful. Will definitely order supplies thru Hardy Diagnostics. Thank you.

blacktara
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Could I ask if Phosphate-buffered saline is also a possible buffer for the wright-giemsa stain or is it preferred to use the hematology rinse solution? I'm conducting a research paper for a school requirement

mono.LK
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The video on the One step stain is confusing, it says you do not need to use buffer, yet you say to put it in the buffer. Very confusing.

GretaHenson-bw
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Tell me the preparation of giemsa liquid before staining

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