How to determine which strand of DNA is transcribed into mRNA?

The template strand of DNA is read in the 3' to 5' direction. The newly synthesized RNA strand is formed in the 5' to 3' direction; it runs in the opposite direction of the template strand. Hence, if the newly synthesized strand is synthesize in the 5' to 3' direction, the template strand is read in the 3' to 5' direction.

And contra another of his claims, the coding strand is not the strand that gets transcribed into RNA, the template strand does. The DNA strand that is not transcribed is called the coding strand because, except for DNA having T and RNA having U, that DNA strand's sequence is the same as the primary RNA transcript's sequence, since they are both complementary to the template strand.

You don't have to take my word for it (even though unlike our person who doesn't know biology but claims to be a professor from Harvard, I actually do have a degree in biology), here's an undergrad cell biology text confirming what I said ...

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“(1) In contrast to DNA replication, where both DNA strands are copied, only one of the two DNA strands – the template strand – serves as a template for mRNA formation during transcription. The nontemplate DNA strand, although not directly involved in transcription, is by convention called the coding strand because it is similar in sequence to the single-stranded mRNA molecules that carry the coded message. … During DNA replication the base A pairs with T, whereas in transcription the base A pairs with U. Hence the sequence of the mRNA molecules is not exactly the same as the DNA coding strand, in the mRNA contains the base U anywhere the coding DNA strand has the base T.

[akhilaunni]

This is for the first time am hearing and learning biology classes in youtube for a long time..Ur explanations r really nice sir..I really respect and love you from the bottom of my heart.Thanks a lot..

[maxra]

Everyone should also read the Text! It is very informative and complete

[shakirarollege]

Thank you so much for your explanation... Lots of love from India

[MohammedAlAli]

Clear and helpful. Thanks from Nebraska, USA

[Jonathan-rfcp]

"the coding strand is not the strand that gets transcribed into RNA, "
The coding strand IS the strand that gets transcribed into RNA. You and whoever you were having a disagreement with are actually on the same page conceptually. You just misunderstand the use of the term "transcribe". "Transcribing" means to copy something, and in this case, the strand being copied is the coding strand. The template strand is *used* to create this copy, but that doesn't mean that it *is* being copied.

[alexleung]

I'm confused. The top strand which you label as the template strand also has the sequence ATG in the 5' to 3' direction, so couldn't both strands thus be used as the coding strand?

[nickandakai]

I'm confused....so the template strand is read from 3' to 5' and you're looking for the start codon GTA (because in RNA from 5' to 3' you're looking for ATG) correct? But in the example strands at the top of this video, both strands reading 3' to 5' have GTA...so what's the difference? Please clarify

[proudindian]

Well explained
Thank you!
Keep it up...

[ibrahimmalki]

I'm confused about how you read the strands to find the start codon because the top strand also has GTA (3rd base-5th base).

[_kunal_]

How strands are used in protein sythesis????

[happyfamily]

so your mean is that mRNA use own "AUG" base to recognize which strand is cording strand, then know which strand is template strand?

[antanariva]

You explain everything very well, but you doing determination based on RNA. Ask the same question without RNA. Before it transcription. I understand that it was not your aim, you did very good educational video. But could you answer on my question: How to determine which strand of DNA =will transcribing= into mRNA? It is very imprortant questions when we speak about heterozygous mutation. Thank you.

[mario]

Knowing that this isn't a complete gene
When you started looking for ATG , why did you look for it in one direction (from left to right), the same as for GTA you only looked for it (from right to left)
If you looked for ATG (from right to left) you'd have found it, but in that case there would be a bigger part untranslated (five prime)

[sabharabaya]

Is it always the 3' to 5' DNA sequence that is transcribed for all genes??

[shethelucky]

can i just ask for clarification, do we use the complementary strand as basis for mRNA?

[eachoneteachone]

(Info given on One parent strand) 5'- TAC- CCA- GGC- AAT-CGC-AAA-CAT-TGG-TCA-ATT-3 (Determine Nnucleotide of other parent strand): 3'- (Determine mRNA that corresponds to the other parent strand): 5'- UAC-

PLEASE SIR. CAN YOU CONFIRM IF MY WORK IS CORRECT.

[kareemmohammed]

Can u please tell me why u take AAA ?
Should I take any sequence ?

[usamajavaid]

Hi can you plz label which strand is
forward/ reverse
coding/non-coding
sense/antisense
template/non-template

[Al-ovqb]

Thanks that’s help
Can u help me with this question

using the DNA strand below non template provided the requested information. DNA 5’ A T G A A T A T C C G T A G T A G T G C A G T A A T C C C G A T T T T T T C A T A T T G A 3’
a. the mrna strand codon that would be necessary for translations.
B. The amino acid sequence that would result from the translation of the mRNA?

[MayorT]

I thought in mRNA, Adenine is matched with Urysole not Thymine with Urysole