HPLC - How to read Chromatogram Easy Explained - Simple Animation HD

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This video explains the concept of the chromatogram. Don't focus on the numbers as the numbers are fiction. It's all about the basic principle of the chromatogram.

Learn the basic concept of High Pressure Liquid Chromatography

01. Introduction

02. The Mobile Phase

03. The Stationary Phase

04. Normal Phase HPLC vs Reverse Phase HPLC

05. HPLC Isocratic vs Gradient analysis

06. HPLC - UV-VIS detection of analytes

07. HPLC - How to read a chromatogram?

08. What is the difference between HPLC and GC?

[Luisam_루이사]

OMG I really love all of these videos 'cause it's helping me a lot for tuesday's final exam <3 My teacher told us to watch these videos in order to understand all of the topics in a better way....and wow he was so right

[Lostpanda]

For those struggling with calculating the concentration for compound X; notice the ratio difference between 200mg/l and 250mg/l, the pattern is that for 1 mg, the peak area increases with 1000. Therefore its only a matter of multiplying peak area for compound X with 1mg/1000... The answer is as stated in the video: 207, 757 mg/l

[y]

Yo dat peak area was THE SHIT, bro!
11/10 would analyze again.

[averymerta]

By far the best video I’ve ever seen for explaining this in simple terms, I now get it, Thank you ❤

[superluvdub]

Wow! Thank you. I wish you were my science teacher

[dr.mohammadasifiqubal]

Your all videos are well explained. I really enjoyed. Keep it up and upload more

[phuonggiang]

this saves my assignment >< thanks a lot

[ayeshasyed]

Loved the way of explanation....hats off...keep up the good work

[atikaadnan]

tqsm for this well explained video. it helps me a lot

[TutunChanda]

Lovely.. most easiest explation. Thanks a lot

[examtime]

Explanation is good
But it’s so irritating to watch video without voice

[rr]

This was very helpful to me. I have a question for you. I need to create a tool (either in R, Excel or python) to calculate and plot the critical peak pair (pair with the lowest resolution) for a given set of parameters. Firstly, what is "resolution" in this context? Also, can you point me to literature that would help me come up with this tool?

[makayacine]

simplicity is the ultimate sophistication

[minalbhalerao]

Thank you for this video. It is helpful.

[NitinKumar-ezcc]

Very good job sir ji
Thank you so much

[heatblast]

super ji I am watching this series of video which is more informative than my book I am preparing for analytical engineering test using this video

[sonison]

Where do you get the retention times from?

[emmanuelmichael]

What else does the peak height indicate if it doesn't guarantee more concentration than any other below jit

[RajeshKrRanjan]

I uploaded practical video on HPLC and HPTLC analysis using software OpenLab and Visioncats...

[jebusslaves]

What does it mean if the baseline is not straight? Is it an error with sample prep, or an error with detector?