Change CT values of qPCR data into Relative expression Graphs

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#qPCR #deltaCTMethod #relativeexpression #howtochange
In this video, I have designed an excel sheet, which can change CT values of qPCR data into Relative expression graphs easily. You can get this sheet for free. The delta-delta Ct method is a simple formula used in order to calculate the relative fold gene expression of samples when performing real-time polymerase chain reaction (also known as qPCR).

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Dr asif ali, dr muhammad naveed, genome wide study are the best channel for all biologist. Highly recommended to all bioinformaticians.

sreeram
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Thanks for creating file of excel for all, i love your videos

keepcalm
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Woaao great sir....i always appreciate your work

Kaleem.a.q
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Very informative knowledge you ever conveying to us.
Keep it up.

FarooqKhan-ghbp
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Thanks for sharing the file. I would really appreciate if you could teach in your next video how did you do your calculation and explanation behind it? I would love to learn.

Mahadihasan-iezv
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How would I further proceed for statistical analysis to check whether the genes expressed here are different significantly using ANOVA??

PraneshKarthick-in
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What are the following column headings abbreviations of?
sdgoi:
sdendo:
deltact:

AmanUllah-tkwt
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Nice video. How can i make the calculation if my gene of interest is expressed more than housekeeping gene? Thanks

judith
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Sir how to add *, **, *** p value with it?

musicaccount
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i think this is the worst explanation i have heard

JohnRobertsPadde