Bio molecules part :-4 sequence determination of peptides

#sanger#edmandegradation#endgroupanalysis#sequencedetermination

Sanger had developed a three stage method for identifying, quantitatively measuring and characterising the terminal amino acids in insulin. This involved treating the protein with FDNB, subjecting it to acid hydrolysis then separating out the coloured compounds with chromatography

Edman degradation, developed by Pehr Edman, is a method of sequencing amino acids in a peptide. In this method, the amino-terminal residue is labeled and cleaved from the peptide without disrupting the peptide bonds between other amino acid residues

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[cricketerkhushichoudhary]

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