Designing of sgRNA Using Benchling- CRISPR Cas9 Gene Editing Technology- Design Your Project 4-B

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Комментарии
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Awesome..please make videos frequently

mominamalik
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Keep it up nice work.. we need some more

aryan
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Very useful .. can you please upload further videos after sgRNA

meetparmar
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Very nice video very informative. Thanks for sharing this. Does the position of the gRNA matters when I want to introduce a point mutation ??? And How do we design the donor sequence for the corresponding mutation?

nola-baissa
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Just a question I have about the acceptable E value. It should be less than 0.05 to be significant? Like the p value?

hanik
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HI..IF you can show final control of Cas9/sgRNA construt and DNA analysis using ICE to Check for INDELS???

AbuBakar-yvqx
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Hey! I didn’t find my sequence listed on Benchling, how to proceed then? If I copy the sequence, where to start ? From the ATG start codon? Thanks !

nola-baissa
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Do you have a video on the entire knock out procedure using Crispr (starting from sgRNA synthesis to cloning and screening)? If so please provide me with the link

ananditabasu
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how can i do it for rice, here only humans and mouse?

mohammadasadullah
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Yeah ok so i need to do some crispr cocaine .... uhhh am i at the right place ?

grigorycitizencook