Benchling tutorial (Recombinant Vector)

Clone cDNA encoding for the protein into a new plasmid for bacterial expression and biotinylation pQEBXRE10 and replace gene PRET10 (red arrow).
Select cloning strategy with suitable restriction enzymes:
- identify enzymes that cut once upstream and downstream the sequence to be replaced -check that enzymes do not cut inside the insert
-design PCR primers (with suitable restriction sites) using Benchling or other online tools

gene sequence:
1 agaagtctca gctacagctt tcacttcatt ctccgctaat taattaatta ttaattaatt
61 aagtctcagc cacagctatg aattcagcta ttttaactgg tttccttttc gtcctccttt
121 gtgtcgacaa catgacttca gccggctcgg gtaaatccgt ggtggccaac cagctgatcc 181 ccataaatac tgccctgact cggatcatga tgaaggcgga gttggtcgcc ccaatgggca 241 tccccgccga ggacattccc cgactagtca gtctgcaagt caacagggca gtgccgatgg 301 gcacaaccct catgccagac atggtgaaaa cgtaccaacc agcgaagtaa ttctgagggt 361 gccaaggagt ttcttcccaa aaccagaaga agaaatgccc cctctcacaa tcaaccttgt 421 ttttgtcaga aacccaagtc tgtccggatg ttaactgaac atgtcaaaac ctgtggagac 481 tatgttgaga tttgatggtc tgaaaagata aagcatataa ataaaatttt gcccaaaaaa 541 aaaaaaaaaa

[maccuswong]

best tutorial video, direct and informative. thank you so much.