how to do circular dichroism || practical biochemistry

cd is a popular technique for determining protein secondary structure. if you have any questions leave a comment.

optimal protein concentration for cd is about 0.2-0.3 mg/ml. If you go higher you will get a noisy tail and if you go lower the signal will not be great.

if you like it subscribe and check this video:

#cd #circulardichroism #labtutorials
#womeninscience #science #protein #chemistry #biochemistry #labskills #practicallabskills #سرکولار دایکرویسم

[fahimasif]

It was really well explained and a nice video. It had helped me a lot. Thank you very much.

[manishachauhan]

I recently did CD and the jasco analysis say it has no beta sheets but when I use k2d2 software it shows beta sheets. any idea why it is showing such variations. thank you. great video btw❤❤❤

[vhahangwelemulaudzi]

Hi Biochemist Melo. Can I please have the parameters that you used

[muhammedyousafk]

simple and humble
thank you @Biochemist Melo

[tamannabachhawat]

Thank you, This was very helpful.
Love from India.💛

[tremeramcbean]

Thank you so for much posting this video! Can you please tell me what concentration of protein was used?

[neginhadisadegh]

Would you mind prepare videos of full process? And things about NMR and x ray crystallography would be usefull. I mean the full protocols in lab

[I..]

It was so helpful...🤩...please continue making videos like this..😊

[tornadoable]

Hi, thank you for this excelent video! I have some questions (probably silly ones). Situation: a experiment where i used a buffer as blank and the software was set to execute baseline correction. Does it means the further spectra obtained from samples will be already subtracted from buffer? Do i still need to click on baseline to manually adjust it after smoothing? If so, how i do the adjusting?

[samueltindell]

How do you read the flow gauge? It Should be about 3 liters per minute correct?

[hanneloreborja]

What would it look like if the protein has a rossmann fold?

[samueltindell]

Were you using a spacer so that the cuvette fits properly and if so does the Cuvette go to the right or left of the spacer? Also, any more videos on CD would be greatly appreciated. It's sort of embarrassing but we have had a Jasco-1500 for about two years and haven't had time to learn how to use it so this video was fantastic.

[biochemistmelo]

Which lab techniques would you like to see next?