Circular Dichroism Spectroscopy for Protein Structural Analysis

Circular Dichroism Spectroscopy is a useful technique for characterization of secondary structure of proteins. Additionally, it can be used to monitor protein structural stability, cofactor binding sites in proteins, and to study protein folding. I discuss the theory and applications of CD Spectroscopy in this video.

[debesray]

Describing using animation was fantastic for a clear understanding, which we miss in the papers or articles. Thank you.

[viktorija]

This really helped me understand this study. I was reading it before I watched the video and I was very confused, and now that I watched the video it is very clear how it all works. Thank You !!

[MajaMika-gn]

found this video very helpful, since there are no studies on CD in my native language and reading an english article provided by my prof (chemistry student here) didn’t make sense to me. thanks a lot! hopefully i’m gonna pass my test thanks to your video

[mthakur]

nice information gathered to talk on CD. Keep it up

[maksimnovikov]

Thanks a tonn!! Are there any other CD resourses you recomend? Especially for cofactor binding?

[ABR_]

Great video. How do I normalise my data by the number of peptide bonds?

[shraddhabose]

very nice! well explained. which buffer do we need for CD run? I think 20-50uL of 0.2-0.5mg/mL concentrated protein is required right?

[samarthkumar]

Sir,
How can we study conformational changes in a protein structure?(Except the conventional techniques we have)

[umarhasnain]

15:31, why is this dotted line inactive?? How will I understand that??

[kajalguleria]

Thank u so much sir..this was best explaination

[kaiser]

From wich article is the figures at 15:15?