Making Petri Dishes

How to make sterile petri dishes with nutrient agar in class cheaply.

[SakkiDuran]

Hey you left me wondering 2:57 why did you put those in there?

[allisonbuhay]

Hi! Just wanted to say thank you for making the video!! It was really thorough and easy to follow, and really helped me out with my science fair project. :)

[riccardocarbo]

I think 15 minutes is the standard using a pressure cooker/autoclave. Regular boiling (not pressured) takes 40 minutes. Thanks for the info! Btw... I wish you'd say "sterile" versus "clean." There's a real difference.

[tracysamuels]

Hi..What is the actual Full recipe for making the liquid agar please..as I don’t see the amounts tht used..( I may hv missed them cause I’m disabled) but I would b v happy to know thanks xxx

[granderondeproductions]

I'm surprised you're not using a still air box! I'm learning how to grow Shiitake mushroom and I'm starting from a Liquid Culture. My first two attempts were completely a bust, but then the next two were perfect and have clean mycelium on them. So I'm a bit excited for that!

[tinkerbell]

It's really helpful for me, thank you, sir!

[gjorgjiristov]

hello is it better if i use 40% normal water and 60% water from boiled yeast and all that mix with agar ?

[leirevail]

What tempeature was the hot plate set to heat up your water in the pressure cooker?

[dezyrides]

why don't people put the agar in the dish and then pressure cook the whole thing?

[eph]

I don't have an autoclave or a pressure cooker. Can I just put my agar mix inside a jar, and then place it in boiling water for some time?

[jvc]

Instant pot is your friend here. Plus multipurpose.

[michelleimage]

Hi, why does my petri dish didn't solidify? I used 1tsp sugar, 1tbsp agar, 1 knorr cube, 1/2 cup of boiled water

[dummkompf]

are you just pouring them in open air?

[TammyOzuna-rnjf]

@bright biology- can I use a UV light to sterilize petri dishes?

[youngchef]

Hello are the styrofoam cups necessary or just an added exparement

[shubhamdhall]

The video was nice but I wish that your camera didn't blur the part when you were filling the beakers with agar.

[ellleHOP]

Hello, it's a helpful video! I have one question about the water though. Can I use water from the tap, or is it a must to make agar with distilled or purified water?
Thank you for your time!

[jessicahewitt]

What is the purpose of the styrofoam cups? I understand the properties changed but not the reason for adding them.

[jerseyhovidea]

Hello, can I pour the mix to the glass petris, pack it to al foil and than sterilize it in pressure cooker?

[funkblack]

I can't identify agar for microbiology in my country. Will any vegetable agar work?