DIY Tissue Culture: How to Get Started for Less Than $200

I sacrificed a finger to bring you this video. please subscribe

(use code PLANTSINJARS for 10% off)

Supplies List Below!

(I am aware that Philodendron Mamei is not technically a "self heading philodendron", but this protocol has worked well for me before for other similar philos!)

Timestamps:
• 1:31 Building a still air box
• 3:18 Tissue culture supplies
• 4:45 What is a tissue culture protocol?
• 5:03 Where to find tissue culture protocols
• 5:44 What is tissue culture media?
• 8:55 How to read a tissue culture protocol
• 11:00 Making tissue culture media
• 13:52 Sterilizing the media
• 17:19 Prepping the still air box
• 18:30 Preparing the explants (tissue samples)
• 19:39 Putting the explant into tissue culture
• 25:30 Conditions for growth
• 26:19 When to subculture

Please note that some of the links in the description are affiliate links, which means that if you click on one of the product links and make a purchase, I may receive a commission. This commission comes at no additional cost to you.

[jorgesantiago]

I do this professionally in a research lab. I’m honestly impressed by your work. Great job and keep it going!

[juandiegosoledadbarajas]

I am a biotechnology engineer and it is wonderful to see this kind of micropropagation content! Let's hope that more people get their attention to this beautiful thing about science!

[Zayskibop]

Mycologist here, been dying to learn plant culture, this is such an incredible series (especially when you have everything you need) 🔥❤️😂

[ShineAqua]

I got an associates degree in horticulture, but couldn't justify travelling to the land grant school for a Bachelor's, so as a certified plant nerd, this is the raddest thing ever. I remember buying a pineapple plant propagated this way from Disney's The Land ride, it made me want to study plants and hydroponics, and I love combining tech and nature. Keep this up, please, and inspire the next generation of plant nerds.

[literallyjustsomegirl]

It was actually great having your fiancee do it live, with your coaching and notes! I feel like you added a lot of really helpful details that someone with experience might otherwise forget.

[guppy]

Seeing you teach someone for their first time - that was actually EXTREMELY helpful and made me feel so much better and more comfortable about attempting this one day

[ultrasoft]

As a biologist, I find your work very-very impressive, the content you share here is amazing, I am stunned! And of course I've just subscribed to your channel :)

[PlantCellTechnology]

Thank you for helping make tissue culture accessible!

[cannabisscience]

Let's go! Great video series. In my plant pathology grad student years, I did tissue culturing to remove viruses from fruits and vegetables in a lab with probably $50k+ gear. Getting this down to $200 to bring the science to the masses is just awesome!

[audreyschilling]

He's trying so hard and its so hekkin cute!! The way you can see every single half error he did (note: yes it was his first time said half errors are borderline expected....) But he was like wait wait fix fix... So wholesome <3

[dragonclaw]

I'm sorry you got injured! I'm a surgeon and i remember the early days of putting blades on scalpel handles in class. Blades were flying everywhere!(especially with brand new handles, they were very difficult) 😂. Nowadays, nurses put them on for me in the OR or we use disposable scalpels in the clinic. Hope you are healing well, glad to see it didn't stop you for long. Keep up the good work! Thanks for sharing.

[greensage]

I am 60-year's old, and a Life-long plant I was around 13, I went into a Woolworth's that had a luncheon area/kitchen/diner. I found a Product Stand with Test-tubes containing clear gel and a small portion of plant growing in it. The Cardboard backing it was attached to advertised colorfully as "Grow anything in this medium", and I was captivated. To me it seemed like incredible Science to do such a thing, ...Cloning! I really wish I bought it, but knowing Regulations in those days, it was probably best I didn't considering the Ingredients you are talking about! :) I might of grown a few extra fingers, and then some! LOL

[jonathanbennett]

I even added gloves to my still air box when I was working with liquid culture making Petri dishes growing mushrooms

[darreneast]

One of the best tutorials I've seen, regardless of subject. No relevant info left out, and only relevant info kept in. Both concise and complete. Thank you for taking the time to both create and share this, it's very much appreciated. Oh, and all the best for your approaching nuptials. May you both have a long and happy life together. Cheers!

[onepiece]

I have worked many years in many labs doing this for strawberry and other species. The fact contamination is not a major issue for you is a miracle since for many people, working with wild plants, contamination is always a problem. Great job

[awnage]

26:06 "Plants are Science". This reminded me so much of one of my grad school classes from 20 years ago. Well done making it accessible to the world.

[LaurenJacksonlocs]

You can also use baking soda to up the PH and distilled white vinegar to lower it to save some extra bucks 😊 I love your videos! Thank you for sharing thorough easy to follow info on PTC✌🏽🌱✨

[debbiefox]

Omgosh!! I am so excited your video showed up in my feed. I love plants and own about 140 houseplants. I love studying botany & science. You really broke this down into such an easy to follow tutorial. Loved this! I have always wanted to do this with orchids. I'd probably also do this with all my Orchids, Hoya, Dischidia, some ferns, Jungle cactus, Zig Zag Cactus and my philodendrons. Thank you for sharing and I am honestly so sorry you got injured badly. A 3 day hospital & severed tendon are a huge deal. I hope you don't have nerve damage & your rehab goes well. So thank you for your sacrifice for Science. I can't wait to binge your other videos abd my inner nerd is super stoked! Take care & get well soon.🫶💚🪴☘️🌵🌿🌱🍃

[TiberiusCowgill]

This popped up in my recommended feed, so glad it did. This content is truly amazing, and I'm shocked you haven't hit 100k let alone 10k subs yet. Keep up the great work, truly amazing and educational!

[michealkish]

After watching other tc channels, and reading plants from test tubes. I like yours the best. In my opinion they do things that seem odd in process. 🤷🏻