The Future of Houseplants: Tissue Culture (TC)

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TIMESTAMPS:
0:00 Intro to Tissue Culture
0:07 What is Plant Tissue Culture?
0:22 Micropropagation is a style of Plant Tissue Culture
0:30 Tissue Culture Process
0:51 What is MS Nutrient Media (Murashige and Skoog )
0:55 Plant Tissue Culture Multiplication Recipe
1:01 What is Subculturing
1:05 Multiplication Phase of Tissue Culture
1:07 Rooting Phase of Tissue Culture
1:18 Acclimation of Tissue Culture Plants
1:22 Tissue Culture Lab Setup
1:26 Laminar Flow Hood for Tissue Culture
1:31 What is the purpose of a Laminar Flow Hood for Tissue Culture
1:40 Tools for Tissue Culture
1:51 Thai Constellation Monstera in Tissue Culture
2:23 HEPA filter for Tissue Culture Lab
2:35 Tissue Culture Demonstration
2:47 Remove unneeded biomass from the explant
3:00 Subculturing for micropropagation
3:50 Sterilizing epipremnum explants
4:35 Sterilizing tools for tissue culture
4:52 Placing explants in multiplication media
5:13 Multiplication of Thai Constellation Monstera
5:40 What is Subculturing
5:52 What finished tissue culture looks like
6:00 Acclimation process for tissue culture
6:20 Plants that are grown from Tissue Culture (TC)
6:40 Advantages of Tissue Culture
6:50 Disadvantages of Tissue Culture
6:55 Rot on Tissue Culture Monstera Thai Constellation
7:05 Advantages of Tissue Culture with Genetic Modification
7:23 How Tissue Culture helps preserve genetics for future generations
7:52 Please Subscribe to the Channel :)

In this video we go over:
What is Tissue Culture
Tissue Culture Explained
The History of Tissue Culture
Micropropagation
Tissue Culture in Houseplants
What is TC
What does TC Mean?

#TissueCulture #ThaiConstellation #RarePlants

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I do earn a small amount money from amazon affiliate links if purchases are made, but with NO extra cost to you. This does not create incentive to make videos like this. My goal is to make reliable, honest, plant content viewers can trust. -Chris

[anushaudayakumar]

I'm A/l agri student so this video is a very important for me.And i like this video .thank u so much❤❤

[ChadGardenSinLA]

I can't express enough how much into the science I am with this hobby! Thank you for making this video. I'm curious to know if the protocols you used are generally available online for "common" plants like pothos. I understand why they are a well guarded secret, but I've seen a lot of small nurseries here in California (as well as instagrammers) performing tissue culture in their back rooms. So it makes me wonder how readily available (or shared) certain protocols are seeing so many hoods outside of a hospital or lab setting.

[northernplantucky]

I think this may be my favorite video you've done so far❤🎉

[alimartinez]

This is fascinating! Thank you for the information!!

[THOTHvii]

Wow, so I have watched about 8 videos pertaining to this subject and I wish I would have just started with this one. This is a very well put together video. I really like your brevity and concise instruction. I think TC is going to be my new hobby. Thanks for sharing the video.

[rj]

Thank you for sharing the knowledge. Blessings to you 🙏🏻

[viralipanpatil]

Thank you so much its very informative and easy to understand

[karenfreedman]

I’ve always wondered…
Thanks for sharing!❤️

[renatecormick]

Amazing, thank you so much for sharing this information, I enjoy learning about TC and the future of using it.

[xvsrjol]

I'm a pharmacy technician by trade and I specifically work in a sterile admixtures lab where I utilize aseptic technique to prepare drugs for intravenous use. I find the process of tissue culturing really fascinating and I kind of want to try it myself since it seems to fall in my lane. I'm a little curious about your setup, though, if you don't mind some questions.

In a typical laminar flow hood, the air flow is in sheets (hence "laminar flow") from a singular direction. I could be totally wrong, but I don't think that can be achieved with the type of filter you're using. We also utilize PPE including (but definitely not limited to) sterile gloves to avoid introducing potential pathogens. I know I'm working with more sensitive mixtures, considering their administration, but do these practices not impact your process?

I'm legitimately curious because I'd like to see if this is something I could set up at home and while a fully sterile environment would be impractical, I could probably rig something up if it doesn't need to be 100%!

[donnawest-newman]

This is great information, thanks for dumbing this down. So interesting

[Eric-yjxg]

Check your flowhood air vortex. Sometimes, the way you mive your air around can create pockets of suction bringing bad air in the flowhokd.

[rosemontez]

Thank you for sharing! What an amazing concept. Good job on this video and detailed explanation!

[Lisa-gswj]

It's nice to see how you explain everything about TC. I myself working on Plant tissue culture where I have to induce callus. I have used reported PGR for callusing but it seems like any hormone and its concentration has no effect on my leaf explant which is concerning. Also I'm using both young and mature leaves.
What could be a reason for my leaf explant to not give any response on any callus inducing PGR?
If you could answer my query?

[queeniefelixjoiedomantay]

Hello . I love your videos. Im really interested to learn more about tissue culture. All the tools and compounds needed. I really want to try it at home.

[nelsonmayor]

excellent video, thanks for sharing.
What antioxidant do you use for monstera? I have had serious problems with oxidation.
Thanks

[OyetOsiris]

What kind of jelly did you use inside the bottle

[plantypittsburgh]

I am really looking forward to the future tissue culture videos. I harbor delusions of my own little lab some day.

Another video topic that you might want to cover is light & variagation. I see conflicting advice and in my experience when increasing light should improve variagation, it does not. (I'm looking at you, cebu blue ghost). I've never had a plant revert. How does variagation happen? How is it replicated in tc and when can it not be? So many questions!

[KamrisAjelico]

Normally I would not post, especially on a video this old, but that setup is not using a laminar flow hood. That is very much going to create turbulent airflow, especially near the edges. It's likely forming a swirling pattern in those areas, potentially pulling air in from the outside. I would be curious to see it tested with a smoke machine or such. Laminar flow is a technical term, it has meaning, it is not just the name of a device. A lot of science and engineering can go into making something have laminar flow.

[lindsey]

could someone answer a question for me? I understand the concept of tissue culture BUT how is it any different from propagation aside from the sterile environment and the more nutrient rich growth medium. I just wonder, if the cutting and everything is all the same, is the only difference a more controlled environment? This may be a stupid question. Lol. I'm just trying to understand exactly how it works and why it's preferred to regular propagation? Especially with plants that aren't too difficult to propagate to begin with. EDIT....maybe I should have finished the video first. Lol. I think I understand a bit better. This was a much more in depth explaination than other videos I've seen. Very interesting