Green Fluorescent Protein | What is this Thing?!

What is this thing?!

Trying to document grad school one YouTube video at a time, from lab equipment to genetics lessons to interviews with other students! Each week is a new view into life as a grad student, and the rollercoaster that is getting a PhD.

Twitter: @AlexDainis
Instagram: Alex.Dainis

Music:
Licensed under Creative Commons: By Attribution 3.0

(All thoughts and opinions are my own and do not reflect the thoughts or opinions of my institution.)

[hoopsonwheels]

Bacterial paintings...art of the future

[منادمنّهلعدّةسنيناوفيخاطريهكلا]

Love your energy and attitude. You just won a subscribe from a fellow content producer. Good job Alex!

[ssthapit]

Short yet helpful introduction. Thank you. Got the information I needed. Lowercase for victoria at 0.15.

[josepshsenekji]

very simple and straight forward video ... good job !

[Kram]

Oooh you participated in the painting challenge, neat, looking forward to it!

[kai]

If you liked what you just saw, check out "brainbow" by Lichtman and Sanes. they used a variety of different fluorescent proteins to mark individual neurons, which is not only fascinating but also beautiful

[jasonmp]

GFP was isolated in the 60s by Shimomura. He won a Nobel prize for it.

[FishyArchaeologist]

Great video! There's an interesting side story about GFP. Doug Prasher is often regarded as having come up with the concept for GFP's modern uses, but his grant to develop it was turned down by the NIH. He ended up leaving academia due to his inability to gain funding, only to have his work on GFP turn into the Nobel Prize winning scientific tool it became (through the work of his lab mates, including Chalfie).

It is a fascinating and terrifying story about the vagaries of grants and academia.

[stephencavey]

I'm a huuuuggggeeee fan of your channel. Thanks teach us about cool science stuff in a way that is very easy to understand. Very cool! Keep rockin' it!

[morganburns]

is this the same as Mneongreen? is this a vaccine ingredient?

[BarbarianGod]

Bacterial Paintings.. now there's a term I didn't think I'd ever hear or want to know more about, but here we are! :P

[Sath]

Do an episode on HRP. I think everyone would love to see that.

[purplepanzer]

Can't wait for your next video :)

[zahraag]

You try to give the video more brightness it will be great if you do

[BruceWayneReal]

Could you do a video on engineering yeast(brewing yeast if possible) to contain this protein or similar protein?

[WarmWeatherGuy]

At 1:00 you say you could use gfp on a gene that has a very clear phenotype. Did you mean to say one that has a very unclear phenotype?

[szczurpiekielny]

theres even GFP variant that fluoresces in infrared range

[supersmashsam]

Really impressive, no wonder this won the 2008 Noble prize in chemistry!

I was hoping you had shown the fluorescence of that E.coli broth in action using some kind of lamp!

Do you use this technique in conjuction with like confocal microsopy to get information on where the genes are expressed?

[IHLWonk]

Should use other proteins to tag, make make a colour spectrum of them.

[beliasphyre]

Does GFP break down at a predictable rate, so you can detect when a gene that was on is turned off?