GFP and its application in biomedical research | Visualizations of cell using the GFP reporter

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Green fluorescent protein (GFP) has gained widespread use as a tool to visualize spatial and temporal patterns of gene expression in vivo.It can be used for various purposes like
1) tagging protein
2) checking promoter strength
3) labeling subcellular structures
4) tracking dynamic processes like cell migration
  1. Animated biology With arpan
  2. green fluorescent protein
  3. reporter gene
  4. gfp fusion
  5. fluorescent reporter genes
  6. fluorescent reporter assay
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Комментарии
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You are a lifesaver. From explaining all the PCR methods to presenting GFP, this is a mine of knowladge for us molecular biology students! thank you!

MiCha-jcgg
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BRAVO! Brilliant lecturer! ... All in a nutshell!!
Thank you, you GFP clear for me.
Suggestion: could you, pls, use normal font styles easy to read🤔

livinghope
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Thanks! this helped me find a new brother

bigmanrake
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Why can't we track the cell dynamics for example the vesicular transport with a fluorescent antibody. GFP and antibodies all attach to the target protein and by following their fluorescence pathway, we will be able to observe their movement. Can you please make this point clearer?

deutschnacharabischemgeschmack
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Hello, I study a bachelor biology and I don't understand something. If we put the GFP sequence instead of the gene body, how does the gene get transcribed? without a gene body? and only with a promotor?

mysterysurfers
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What about resource competition between GFP and Protein of interest to effect translation/transcription efficiency

Jammoud
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Sir can we use this GFP to study the formation of prions in nervous system?

soumyadipsinha