Lecture 7b: Flow Cytometry

UCSD Extension School: Applied Immunology (BIOL-40371)
Summer Quarter 2021
This lecture provides an introduction to flow cytometry techniques used for immunophenotyping both basic research and clinical patient samples. This lecture includes a discussion of sample flow plots used to diagnose various clinical pathologies, including B cell acute lymphoblastic leukemia (B cell ALL), severe combined immunodeficiency (SCID), perforin deficiency, and chronic granulomatous disease (CGD).

All figures are either from Janeway's Immunobiology (9th ed.) where noted, or my own original figures.

[izzyp]

thank you, your classes are amazing and extremely well explained.

[muik]

@Annelise, your presentation is clear, concise and very well presented. Thank you!

[Adam-vgpu]

Great lecture! I feel better prepared now for tomorrow's in-course assessment!

[solanohernandezjoseantonio]

Does anyone have any idea about how to calulate the Mean Fluorescence Intensity? I am using a Sysmex Ploidy Analyser.

[rangeo]

Can we apply two different lazers (e.g., FITC and PE) at the same time to excite cells, and then detect and sort only cells that are exicted by both lazers, and get data from computer?
This would be the same effect by Image J. (e.g. Getting FITC and PE images, respectively from fluorescence microscope and then merge them, which generates yellow pseudo color)

[robbysmith]

Hello is there a class I can take up for flow cytometry

[adrianfiliberti]

Thank you Dear Annelise, super clear!, thanks again!