Virology Lectures 2020 #8: Viral DNA Replication

In this lecture we reveal the mechanisms of DNA replication, including how origin-binding proteins recruit the host synthetic apparatus, replication by strand displacement or a replication fork, solving the 5'-end problem caused by RNA priming, and how viral genomes ensure that cells are actively duplicating their DNA to provide access to the DNA synthesis machinery.

[ameensalehyahyaalyousuf]

Yes finally Professor you started using the mouse while explaining the lecture, I got focused in each point you mentioned because of that. I love you Professor

[David-zyst]

Excellent instruction Dr. Racaniello! Are slides available for detailed inspection?

[sjdjjdaaasd]

at 44:55, how did the nick get made at the circular dsDNA genome?

[johnoliver]

If you could engineer a virus with disabled RNA could it be injected so the receptors are blocked and active viruses would be unable to access cells..

[johnmatrix]

Thank you. Some interesting "chemical mechanics" going on here. Is the delivering or connecting arm that is away from the main molecule (at about 41:30: ) "out there" to reduce any magnetic fields coming from the bigger bit of the family particle? And at the same time, it acting as a pipe.

[anthonyroberson]

Explaining me in replication what all can go wrong

[paulnorton]

one question, from me, because I am a bit slow on this. is the DNA is literally manufactured ( using the organic compound ) or is it converted ( DNA A to DNA G e.g) to suit the virus needs to replicate? Since we all carry virus DNA within us I am surprised that no one mentioned this helped the virus to replicate AND help our immune system to destroy the virus.

[johnmatrix]

Thank you. Some interesting "chemical mechanics" going on here. Is the delivering or connecting arm that is away from the main molecule (at about 41:30: ) "out there" to reduce any magnetic fields coming from the bigger bit of the family particle? And at the same time, it acting as a pipe.