Gel electrophoresis Technique

🔬 Discover the Basics of Gel Electrophoresis Technique! 🧬 | Aladdin Creations

In this video, we dive into the fascinating world of gel electrophoresis! 🌌 Learn how this essential laboratory technique separates DNA, RNA, and proteins based on their size and charge 🧪. Perfect for medical laboratory students and scientists, this quick guide covers all the basics, from setup to results! 🎓 Whether you're studying biotechnology, genetics, or molecular biology, gel electrophoresis is a must-know technique! 🚀

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📌 Key Points:

How gel electrophoresis works ⚙️
Understanding DNA, RNA, and protein separation 🔍
Practical applications in lab settings 🧫
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Here restriction enzyme has already been used to cut the dna molecule at specific sites and the small dna fragments needed to be cut out can be separated from the dna molecule by the use of physics. Electric field is applied to the agrose gel containing plate and the dna is inserted in it. The smaller dna molecules(negative) move towards the anode( postive) at a faster rate due to size difference. Like this the required cut dna segments are extracted first. Use of ethidium bromide and uv light is shown so that dna is visible.Then the cut dna are removed from the gel by the process called as elution and can be used for joining with vector dna ( sticky ends concept) with the help of dna ligase enzyme.