Phytoestrogens Screening by Estrogen Receptor and Reporter Assay | Protocol Preview

Screening for Phytoestrogens using a Cell-based Estrogen Receptor β Reporter Assay - a 2 minute Preview of the Experimental Protocol

Emily M. Chester, Emily Fender, Michael D. Wasserman
Indiana University, Department of Anthropology;

We have optimized a commercially available estrogen receptor β reporter assay for screening human and nonhuman primate foods for estrogenic activity. We validated this assay by showing that the known estrogenic human food soy registers high, while other foods show no activity.

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