Klenow Fragment

Klenow Fragment lecture - The Klenow fragment is a large protein fragment produced when DNA polymerase I from E. Coli is enzymatically cleaved with the aid of the protease subtilisin. First stated in 1970, it retains the 5' → 3' polymerase exercise and the three’ → 5’ exonuclease exercise for removal of precoding nucleotides and proofreading, but loses its 5' → 3' exonuclease activity.
This video lecture will teach you the structure of Klenow fragment and the use of klenow fragments in molecular biology experiments.

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[niranjanarajmohan]

Great Had used it while preparing for my NEET exam... Now it's helping me with MBBS!!! Thanks Sir... U r amazing!

[bernagenc]

Still there’s no other video for klenow fragments that describes this well. Thank you for explaining it as simple as possible 🐛

[paramitaadhikari]

Your videos helps a lot in making clear concepts of biology.. Keep making more videos

[mdshuvo]

I am a student of Biochemistry and Molecular Biology from Bangladesh...Your videos makes my task more easier..really u r genius

[kapushkapolis]

Thank you soo much for this explanation. I swear you are explaning more clear than my professor. <3 Lots of love from Turkey!

[abritanath]

Better explanation than in books, thanks!

[apoorvanelli]

Just a small correction: Exo klenow fragment retains the 5' to 3' polymerase activity not the 3' to 5' exonuclease activity. Mutations can be introduced into the genes of the klenow frament to retain only polymerase activity and no exonuclease activity which is termed the exo klenow fragment. :)

[snehaltonde]

You are such a great helper to us.
Thanx for making all subjects of biotechnology much easy!! 🤗🤗

[martinjames]

Hi Shomu,
I think your videos are really great and very helpful. Thank you for making them and uploading.

[ananyachakraborty]

My heart filled gratitude for this lecture Sir

[fekadukorsa]

Wow...Wonderful explanation. I got a clear understanding of Klenow fragment.

[KathyChanHeyHeyYou]

Clear, concise, straightforward explanation. Thank you.

[zeinebelbar]

Thank you it's really helpful IAM so grateful

[heerachoudhary]

Your video is very helpful for me
Thank u so much sir

[fabiolatare]

DNA polymerase 1 removes primer not Okazaki fragment (1:38)

[mayukhroy]

Sir, your explanation was truly helpfull.

[isurithakshila]

Excellent explanations👌best of luck from sri lanka😀

[Abhinav-kfwd]

Thank you so much sir for the awesome explanation.

[viktorsedlmayr]

basically the klenow fragment has the same activity as DNA Pol III, so why not using DNA Pol III in the first place?

[riyawasnik]

Thanks for making the video, it was helpful 😇