Prime editing (another way to edit genes)

So, I don't know why I hadn't already made a video on gene editing as it is a topic I know quite a bit about, but anyhow, there is one now :D. This video will look at the new technology to perform precise genome editing, prime editing and will look at how the method works and how it differs from the canonical CRISPR-Cas9 system.

[DeenyCat]

Starting at a new lab without the best background in genetics and this was soooo helpful! thank you !

[wusam]

I like how you give really great, thorough introduction and that you explain the paper really clearly with many visual aids. :)

[BillKonstantakos]

Great review! Also worth mentioning that they designed sgRNAs that match only the edited strand to minimize double-strand DNA breaks (PE3b); this way nicking of the non-edited strand occurs after edited strand flap resolution.

[fazelamirvahedi]

That was really nice and well-explained. Thank you for sharing your knowledge and skill. Please make more videos like this.

[chun-yinghuang]

Thank you for making such a great video! I’m working on gene editing and this video really helps!

[inquisitivebiologist]

This was an actually amazing video explaining Prime editing. Thanks a lot, you are great!

[videofancrazy]

Really well explained, and you posited all the questions we need to ask about this technology. Your sense of humour certainly doesn't hurt lol.

[aoihana]

Excellent and easy to follow summary🎉

[sanja]

Super good explanation! Thanks for the video and the references :)

[shiyiyin]

4:36, regarding base editing, ABE and CBE are now nickase based as of 2024, i did remember early on they were dcas9;
Cas9n means N terminal fusion protein, can be confused with nCas9, nickase cas9
Great video!!!

[treewx]

So, in Prime Editing, that Cas9 attaches to the Target Strand, but edits the Non-Target strand?

[shroomwave]

Thank you for this video. I'll give you lots of credits in my presentation!

[shikhas]

I cannot stop laughing at the "ooh exciting" at 8:45. Also this video is super helpful :)

[ٹیڑھا]

Thank you so much. This was very helpful

[Mohammadssalehi]

"Ooh exciting" lol Great job!

[BFS]

This is cool, thank you Eleanor, for the animated cartoon video, which helps a lot. BTW, what program you used for annotation and drawing?

[RadwanDerbala--]

A pretty awesome video, no more no less

[MrsSolvinden]

I'm a student currently working on an essay based on the mentioned paper and randomly ended up watching this video. This was really helpful, perfectly explained and easy to understand. Thank you for your work! Not only for the great sum up but also for the critical point of view (like in Jonathan Wilde tweets). 
Ps: Could anyone share what's the previous cell paper he mentions?

[lilyz]

Very well explained!!! I loved it thank you very much!

I think you might've mislabeled two 5’ ends on the pegRNA at around 9:12 minute mark?

[kiara_l]

Thank you so much for making this! A quick question, is the primer binding site in the linker region between the cas9 and the reverse transcriptase?