PRECISION EDITING USING CRISPR - GENE EDITING EXPLAINED!

This series of short presentations on gene editing is brought to you by Dr Adam West, College of Medical Veterinary and Life Sciences at the University of Glasgow, Scotland.

This presentation describes the use of the following three strategies for precise genome editing with CRISPR:
4:13 Homology-directed repair
8:32 Enzymatic base editing
11:34 Prime editing using reverse transcription

Zinc finger nucleases (ZFNs)
TALE nucleases (TALENs)
CRISPR adaptive immune systems
Using CRISPR/Cas9 for genome editing
CRISPR specificity

This presentation is primarily aimed at university students, researchers, clinicians and journalists interested in fields related to molecular biology and genetics. It is for education purposes only.

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More accessible presentations for a wide audience can be found in the “Explained Simply” section of this channel in the near future.

Artwork is © Adam West with the exception of images and data taken from publications referenced in these slides. References to reviews and journal articles are denoted by circled numbers at the bottom right of the slides.

Links to these numbered articles are listed below. We recommend these as your best way to begin further reading on this subject.

Publication List
30. Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG.
A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells.
Stem Cell Reports. 2016 Sep 13;7(3):508-517.

34. Komor AC, Badran AH, Liu DR.
CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes.
Cell. 2017 Jan 12;168(1-2):20-36.

37. Hess GT, Tycko J, Yao D, Bassik MC.
Methods and Applications of CRISPR-Mediated Base Editing in Eukaryotic Genomes.
Mol Cell. 2017 Oct 5;68(1):26-43.

38. Gaudelli NM, Komor AC, Rees HA, Packer MS, Badran AH, Bryson DI, Liu DR.
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.
Nature. 2017 Nov 23;551(7681):464-471.

39. Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR.
Search-and-replace genome editing without double-strand breaks or donor DNA.
Nature. 2019 Dec;576(7785):149-157.

Music credits
“Go, Icarus! Go!” by The Whole Other
“New Year” by Bad Snacks
(YouTube Audio Library)

[StrayBerserker]

Thank you very much for the informative overview of this new technology.

[uquantum]

Terrific presentation that clearly compares and contrasts prime editing with base editing and homology-directed repairs♥

[GroovyGeek]

By far the best explainer of prime editing I have seen so far on YouTube

[gregsmith]

I'm going to have to watch the Prime editing part 10 times more to understand it. It's simple yet complicated.

[emileachou]

Very good explanation. Thank you !! and hope that you make more content about crisper technology and it’s variants .

[courtneyczb]

Thank you for the clear explination :p

[wazhi]

Thanks for valuable content. It seems prime editing could do more than base editing, any case that base editing could be a better choice than prime editing? If not, why base editing is needed then? Thanks.

[chillaxTF]

Sorry to be pedantic, but 1:31 confused me.

If a transition mutation is pyridimine <--> pyridimine or purine <--> purine, wouldn't it involve A <--> G or C <--> T?

Additionally, if a transversion mutation is pyridimine <--> purine, wouldn't it involve A <--> T, C <--> G, A <--> C, or G <--> T?

Please let me know if I'm misunderstanding something here. Great video overall, thank you. You're a great teacher.

[erfanir]

Great talk but very low quality of the slides. Hard to read. BYW easy to follow from your excellent presentation.