Introduction to epigenetics - Learn.OmicsLogic.com

Epigenetics refers to mechanisms of gene expression regulation that do not involve changes to the underlying DNA sequence. At least three systems including DNA methylation, histone modifications and non-coding RNAs (ncRNA) are considered to play fundamental roles in epigenetic regulation.

Epigenetic regulations play an important role in a variety of human disorders and diseases. In addition, age, environment, lifestyle, and other factors influence epigenetic states.

Epigenetic regulation of gene expression has been linked to discrete mechanisms that affect the stability, folding, positioning, and organization of DNA. The most studied of these mechanisms includes DNA methylation and chromatin remodeling, which work synergistically to organize the genome into transcriptionally active and inactive zones.

To better understand the bioinformatics approaches to studying the epigenetic changes in cells, it is firts important to understand the biology and the molecular assays that are used in researching these regulatory mechanisms.

0:00 Introduction
2:34 Epigenetics is
2:48 On the Way From Code to Function
3:45 The Epigenome: DNA
4:37 DNA Methylation
6:11 Histone Modification
7:42 Chromatin Packing
8:23 What Regions can be Affected?
9:02 1. ChIP-Seq: Immunoprecipitation
10:15 Analytical challenges: ChIP-seq
11:11 2. Whole Genome Bisulfate Sequencing
11:30 Analytical challenges: WGBS

[westfield]

Much respect to the Brilliant scientists throughout history who figured out all of this that can’t even be seen

[krishnakumarganta]

Holy.. He is not a biologist by education but he is the best at what he teaches. Thank you Elia

[RockerProf]

Thanks for this video. BTW, those little beads/bumps are called "nucleosomes, " which consist of 8 histones and 146 wrapped DNA bases.

[JesperKleifert]

At about 4.47 you refer to cytosine as an amino acid, maybe just ad a square saying "nitrogenous bases".
Great clip! Thank you

[swissever]

If you call that an intro to epigenetics, than i think i need an intro to the intro😉

[AffectiveApe]

I believe it is bisulfite, not bisulfate, sequencing (someone correct me if I am in error here), but otherwise this is an excellent video.

[ai-uruv]

Thank you, this was one of the best explanations I have ever seen

[stephenleighton]

can anyone explain or give clarity on the following ... does any change to typical Purine & Pyrimidine, folate, B6 or B12 pathways during pre or early post natal periods result in heritable genetics that could be passed from that baby should they procreate in later life?

[UHFStation]

Any animations of epigenetics transfers when cells divide?

[mikejones]

I have a question that isn't quite related to epigenetics but more to something you mentioned-- You said that Chromatin Packing has a governing effect on processes including apoptosis. How could this be the case? Do cells willingly undergo apoptosis or is it the same thing as lysing the cell itself? I've only heard the word apoptosis used in the context of osmosis from a hypotonic(I think) solution being an environment in which cells will explode.

[RockerProf]

@ 8:42, the 3'UTR is not part of an exon. Exons end at the STOP codon. The 3'UTR is after the STOP codon. Where did that graphic come from?

[trevaush]

A bit of confusion in that it is not clear which processes are naturally occurring and which are done in the lab.

[kyrgyzsanjar]

Very nice explanation, stealing some ideas!

[ggbrother]

I have a project about dna methylation epigenetic control of gene expression can you help me about I get information about this topic any reference

[genetilocos]

I have a question that is making me sick. I thought that methylation should always be interpreted as a repressive mark. So, how is it that H3K4me3, H3K36me, and H3K79me for example could lead to gene expression? thank you

[LACERENZANUTRITION]

👍🏼💪🏾👑 Top thanks. Good morning, a question please, the methylation and ipermethylation of the DNA can stopped celular neoplastic? SAM ( S- Adenosil Metionina) is it useful, is it good to take 400 mg every day to silence the transcription of cancer? thank you very much.
Andrea Lacerenza

[boydhooper]

With respect that’s not an introduction. That is a technical overview. Please label things for what they are to avoid wasting people’s time and creating frustration

[SuperElwira]

Do the mRNA vaccines can change metylation? SPIKE? Wot group B we know is needed to metylation and after vacciness people have some neurological shmptomes which was gone after wit B and D suplementation? CRISP/Cas and SPIKE is there any bond? Is tha posiible somehow that ant virus can chamge DNA like we observe in plants world- viris can change colour of the leaves or fly wings?

[AlvaroALorite]

I was under the impression that it was recently discovered that methylation has inherited to some extent.

[misssalma]

I like to make my speciality epigenetics and I really have no idea which program its part of I didn't finish my high-school yet and interesting these things plz anyone can direct me which courses and programs should i take and which faculty should i join so i can take epigenetic as my major ?? thanks you