How to perform PCA on single-cell RNA-Seq data in three simple steps

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References:

  1. Florian Wagner
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Комментарии
Автор

Nice summary for reasoning behind workflow! I was looking for an example of PCA but only saw t-SNEs

mikelawrence
Автор

Really great video, thanks a lot for explaining PCA so well in such a concise way

黄梓煊-gu
Автор

Thanks for the great video. I have a question regarding scaling step; in the scaling step as you mentioned we shouldn't multiply by like a million and the number is median/total umi count. In Seurat NormalizeData function does divide the count by total cell count and multiply to 10000 scale factor. I was wondering what is the difference?

pariaalipour