can RT PCR test be false positive

No

[terryrobinson]

“The first thing to know is that the test is not binary, ” he said. “In fact, I don’t think there are any tests for infectious disease that are positive or negative.” The next part of his explanation is lengthy and detailed, but let’s push through: “What they do is they take some kind of a continuum and they arbitrarily say this point is the difference between positive and negative.” “Wow, ” I said. “That’s so important. I think people envision it as one of two things: Positive or negative, like a pregnancy test. You “have it” or you don’t.” “PCR is really a manufacturing technique, ” Crowe explained. “You start with one molecule. You start with a small amount of DNA and on each cycle the amount doubles, which doesn’t sound like that much, but if you, if you double 30 times, you get approximately a billion times more material than you started with. So as a manufacturing technique, it’s great. What they do is they attach a fluorescent molecule to the RNA as they produce it. You shine a light at one wavelength, and you get a response, you get light sent back at a different wavelength. So, they measure the amount of light that comes back and that’s their surrogate for how much DNA there is. I’m using the word DNA. There’s a step in RT- PCR test which is where you convert the RNA to DNA. So, the PCR test is actually not using the viral RNA. It’s using DNA, but it’s like the complimentary RNA. So logically it’s the same thing, but it can be confusing. Like why am I suddenly talking about DNA? Basically, there’s a certain number of cycles.” This is where it gets wild. “In one paper, ” Crowe says, “I found 37 cycles. If you didn’t get enough fluorescence by 37 cycles, you are considered negative. In another, paper, the cutoff was 36. Thirty-seven to 40 were considered “indeterminate.” And if you got in that range, then you did more testing. I’ve only seen two papers that described what the limit was. So, it’s quite possible that different hospitals, different States, Canada versus the US, Italy versus France are all using different cutoff sensitivity standards of the Covid test. So, if you cut off at 20, everybody would be negative. If you cut off a 50, you might have everybody positive.”

[rdsvrs]

Wrong information provided by you. RT pcr can be false positive due to contamination errors and loading errors.

[Cittamatra]

Get to the point.

PCR uses two primers, that anneal to two specific zones of the target genome. The primers are only 20-30 nucleotides or bases long. The low number of nucleotides on the probes renders the PCR test's specificity extremely low in an un-purified sample.

In short, the PCR test protocols won't differentiate between SARS-CoV-2, influenza A & B, or other genetic garbadge, floating around in your snot sample.

Turn up the temperature cycles > 40 and the number of FALSE POSITIVES will rocket.

Verdict:

FRAUD

[Roshalpha]

It can come false positive due to technical and handling error (example: cross contamination of samples from pipettes and tips).

[Brilliantindians]

False positive bhi aata hai aaj ka 22 June rajasthan patrika pad lo sir ji

[ahmerkhokhar]

Pcr is 2nd time positive after 10 days isolation period. Am i still infectious/contagious? Can i further isolate or no

[ilovekirati]

False information sir, sorry to say one of my brother test positive in pcr kit but negative in truenet machine in same day. What you say about these.

[rdsvrs]

Dr. Please brushes your concept about interpreting diagnostic test

[sandhweerai]

Sir my RT PCR test is equivocal...what that means ??

[kaus]

40% test fake hai negative hoti hai phir bhi paise ke liye admit hona hai

[MintuRajkhowa]

Jese Police Sarkar ke Khilaf kabhi nahi ja sakti Mumbai and UP ka example kafi hai samaj ne ke liye, waise hi Doctors WHO ya ISMR ke khilaf kabhi nahi bolengee kiyuki unka license cancel ho sakta hai.

[supriyakumari]

Sir mujhe 12 apr ko corona ke sare symptoms the fever, loss of taste and smell, cough, vomit and weakness but home sampling nhi horhi thi to us time quarantine kiya home pe aur normal multivitamin liya..today after 23 days maine rt pcr test krwaya meri CT value 10 aayi h.. Par mujhe koi symptoms ni h 2 week se fever nhi h weakness nhi h spo2 99 hai 6 min walk test ke baad bhi.. Taste smell wapas aagya hai just halka irritation hai in through.. Is this normal or false report aayi h

[rajivmangrulkar]

What to do to check false negative, how to confirm, in case if tests done for contact tracing for likely contact with asymptomatic carriers and spreaders and test not done based on symptoms of shortness of breath, taste, odours lessness, fever, cough, cold. How many days after negative test, we must be watchful and can we attend duty.

[anjukumarianjukumari]

Sir agr rapid antigen positive ho aur rtpcr negative to konse test pe viswas kre please reply

[yashgupta]

Okay i am an engineer so I can't say the an well educated and infact well experienced doctor is wrong but I remember on April 3 I was tested positive (reason for testing I had to go somewhere) so it was asymptomatic even after a month on 7th May it was still positive so idk how's that possible I didn't have any problem then too

[AmanSingh-sjip]

Rt pcr test kit ka jakar user manual padhie ek baar

[amanaayan]

Is it possible that rt pcr test shows positive if u have antibodies developed for the covid19 virus??

[udayrajvelhu]

RT PCR is not for diagnosis sir, that clearly mentioned on manufacturers manual . don't make us fool

[somnathgupta]

Sir can RTPCR report show positive dur to vaccination?

[karamchandchadimal]

Kya antigen test false positive ho sakta hai