Extraction Of DNA🧬 From Banana 🍌 Experiment | DNA Extraction Experiment #sciencepur34

Extraction Of DNA🧬 From Banana 🍌 Experiment | DNA Extraction Experiment
#sciencepur34

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👉 Important Informations in this video 👈
Basic Isolation Procedure:-
There are five basic steps of DNA extraction that are consistent across all the possible DNA purification chemistries:
1) disruption of the cellular structure to create a lysate,
2) separation of the soluble DNA from cell debris and other insoluble material,
3) binding the DNA of interest to a purification matrix,
4) washing proteins and other contaminants away from the matrix and
5) elution of the DNA.
🧫 Creation of Lysate:-
Cellular disruption is accomplished with a variety of agents that disrupt cell membranes and denatures proteins. Chemicals commonly used include detergents (e.g., SDS) and chaotropes (e.g., guanidine salts and alkaline solutions).
Using ice-cold water and ice-cold alcohol will increase your yield of DNA. The cold water protects the DNA by slowing down enzymes that can break it apart. The cold alcohol helps the DNA precipitate (solidify and appear) more quickly. Make sure that you started with enough DNA.
🔴What is the purpose of DNA extraction experiment?
The ability to extract DNA is of primary importance to studying the genetic causes of disease and for the development of diagnostics and drugs
🔴 DNA isolation is an extraction process of DNA from various sources.
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