Getting Started with Whole Genome Sequencing - #ResearchersAtWork Webinar Series

Want a deeper and more complete picture of the genome? Need to identify potential disease-causing variants? Studying a novel organism where no genome information is available?

Whole Genome Sequencing (WGS) can help you achieve all of this and more. Since the advent of Next Gen. Sequencing in 2008, Whole Genome Sequencing has been unlocking the mysteries of the genome with unprecedented resolution!

In this webinar, you'll get an introductory overview of how WGS works as well as key considerations to keep in mind when setting up your WGS experimental workflow. To end off the webinar, we'll also show you how to verify your plasmids using NGS!

You'll learn about:
• A brief introduction to Next Generation Sequencing
• Important things to consider when designing your Whole Genome Sequencing experiment
• Understanding the WGS-Seq workflow, analysis, and interpretation
• Other applications: Plasmid Sequencing & more

Duration: 30 minutes + Q&A

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[abmgood]

Useful timestamps:
0:00 Introduction
2:28 A Brief History of Genetics
4:34 Sanger Sequencing vs. Illumina Sequencing
8:40 Intro to Next Generation Sequencing
15:04 Important Considerations for Whole Genome Sequencing
17:04 Understanding the Workflow
27:00 Other Applications: Plasmid Verification, mtDNA-Seq
28:35 From the Human Genome Project to Today
30:14 Additional Resources and Conclusion

[ahmedg.hussain]

This is an extraordinary and helpful webinar! It is by far the best I've seen on NGS and WGS! And I like the way the two presenters switch with their presentations! Well done to the team! The next content I would love to see (I hope I'm speaking for many viewers) is some insight into the analysis of the sequence data. Thank you!

[CriticalTechReviews]

Very helpful for a novice like me. I love your videos explaining the science so I know what I need, and your comprehensive services. I hope to get my project off the ground with your help soon.

[AmjadAli-zzre]

Welldone very helpful webinar regarding WGS, please arrange webinar on Multi locus sequence typing

[dnaseeker]

Very helpful video, thank you. You talked about mitochondrial DNA at the end of the video. But is the mtDNA sequenced along with the nuclear DNA during whole genome sequencing or do you have to make another run ?

[bohe]

Can you add subtitles to the video？This will make it clearer and easier to watch.

[jeanettewong]

What strand does the fluorescent synthesis strand complement to? The reverse strand??? Once the fluorescent base is read do we have to infer the complement nucleotide to get the original template sequence?

[carlettagoodrich-mann]

Lifestyle inherited structures can be modified. GENE DNA mutations can define systems functionality . SEQUENCING gene nucleotide Advantage’s could be the biological answers to creating a new recombinant for Corona . DNA platforms unveil the WHOLE genome that needs to be read. THANKS for the CME review

[jakubzahumensky]

What is the point of the DNA forming bridges like this? And how are the bridges then disrupted for the chip to look as it does in fig 5?

[michaelqi]

But what define the "sequence" ? Is it in 3D, 2D or time space? in other words, if one read AGT, what made that special order A-G-T? Why cannot it be read out as T-A-G ?

[aniellanazir]

Hii like if we want to sequence whole genome of human like 50, having covid 19 symptoms half of them and half of them are asymtomatic. Which seq technique is good for them

[thaileonglai]

How does RNA contamination degrade our DNA samples?

[Raul]

I had to listen to it over and over again the figure out that she was saying "throughput" around 5:00
Maybe clean up the narrative on these videos, people shouldn't have to struggle to understand.

[ekapurwanti]

Assalamualaikum mohon ijin kami Jalasenastri Ny.eka Agus Riyanto mohon ijin bergabung'🙏

[advancedcentercapacitybuil]

the voice of the speaker (in the start), her tone of voice and her pronunciation is really very annoying. addition of subtitles would be very helpful.