Inside a CRISPR Lab

At UC Berkeley, CRISPR researchers are developing better gene-editing enzymes and more efficient delivery into tissues. These innovations will be used to improve the effectiveness and lower the cost of life-saving therapy for genetic disease. Take a peek behind-the-scenes to see what goes into a human genome editing experiment.

Produced by Marica Petrey and Leah Worthington
Cinematography by Mogli Maureal
Edited by Marica Petrey
Sound and color by Mogli Maureal
Featuring Lorena de Oñate, Ph.D. and Rima Mendonsa, Ph.D.
Special thanks to Megan Hochstrasser, Ph.D., Innovative Genomics Institute, UC Berkeley

[huntingkc]

So whomever did the recording and editing did a great job

[peters]

Thank you for the video! On a side note, I think this process would be an great candidate for automation. It seems that the human has to complete soooo many steps...

[farahbourek]

Can you please give me the details about methods of extraction that you use to get crispr cas 9

[zqxzqxzqx]

I've been fascinated by CRISPR Cas-9 since it's inception. I follow all the news I can get, and have read Code Breaker (which was more interesting and informative than I expected!) I'm excited to see it already restoring sight and correcting sickle cell. Thanks for this look into a lab.

[farahbourek]

Please can you give me the methods of extraction that you use to get crispr cas 9. Thanks

[Aztorak_]

Now to unveil my superior alternative: CRUNCHR. In all seriousness though this is excellent.

[SixOhFive]

You can buy a plasmid with pcas plasmid that has antibiotic resistance gene and cas9 gene

[jibraankadri]

very well filmed and documented. great job.

[leo_amorim]

Excelent job on this video. I was studying crispr theoretical part and this video helped me to understand it pretty well. Thanks!!

[sandeepgolui]

Excellent to see...thanks for sharing madam

[mockondo]

Does this lab sell the purified Cas9 protein? Can it remain active through several days of shipping?

[eukaryotic]

How is the guide RNA made? does it have any special feature or is it just extracted mRNA?

[mariadelcarmenmendoza]

Great video!!! She's so sweeet :3

[mrnerd]

Thank you for the video ❤️
Learned a lot... Liked your way of presentation

[ferryjap]

Can i order CRISPR and deliver to out of your country?

[justanotherguyful]

It doesnt actually explain the entire process. A lot of the stuff theyre doing looks like it can be done in anyones garage. Very simple process its just that no one has a video actually showing the real lab work from start to finish. If someone made a 20 minute video actually detailing everything from start to finish then anyone could be a genetic engineer if they purchased the right tools.🤓

[SixOhFive]

I did crispr in my bio class recently we turned a white colored yeast pink

[shin-ishikiri-no]

I get that this can be used for life-saving gene modification, but it can be much more than that.

[Tina-bosf]

I thought I saw a comment saying this but couldn’t find it. Could you PLEASE figure out the code sequence for the vaccine, then reverse it and fix us??? I’m in California. I’ll be knocking on your door soon. I was 49 when I took it. I’m 51 now. I had heart pain, dizziness, almost passing out and no more periods. Please fix us.

[joyaborthakur]

Please Scientist 🙏 Try to reverse the biological aging process in humans.. And also to eliminate age related diseases..