CRISPR Proteins: Enabling the Next-Generation of Molecular Diagnostics

Presented At:
Molecular Diagnostics Virtual Event 2019

Presented By:
James Broughton - Senior Scientist, Mammoth Biosciences

Speaker Biography:
Dr. James Broughton is a Senior Scientist at Mammoth Biosciences. James attended Cornell University for his bachelor's degree in Biology, where he began his career long interest in RNA binding proteins. He then went on to receive his doctorate from UC San Diego. During his graduate work, James applied CRISPR/Cas9 genome editing in the nematode worm C. elegans to investigate the function and specificity of microRNAs. For his postdoc, he joined the lab of Dr. Howard Chang at Stanford University. At Stanford, James examined role of RNA modifications on a newly discovered class of RNA, called circular RNAs. After his postdoc, James brought his expertise in RNA biology and CRISPR systems to Mammoth Biosciences where he works on developing and evaluating new CRISPR-based diagnostic technologies.

Webinar:
CRISPR Proteins: Enabling the Next-Generation of Molecular Diagnostics

Webinar Abstract:
Mammoth Biosciences is developing next-generation CRISPR platforms to build rapid, point-of-care molecular diagnostics. Our innovative DETECTR technology leverages novel CRISPR proteins whose unique biochemical properties make them well-suited for diagnostic applications . By simply altering the nucleotides in their guide RNA (gRNA), CRISPR proteins can be programmed to find and cut any matching DNA or RNA sequence. These unique CRISPR variants additionally interrogate target nucleic acids with single-nucleotide specificity, and they then function themselves as the catalyst for real-time signal detection. The simplicity of CRISPR has already revolutionized the field of gene editing, and is now enabling a new class of diagnostics. Mammoth Biosciences leverages our diverse CRISPR protein portfolio to build a diagnostic platform that is readily scalable to detect any nucleic acid biomarker of interest.

Learning Objectives:
1. Develop a general understanding of the diversity of CRISPR-Cas systems
2. Understand how CRISPR-Cas systems can be used for diagnostic applications

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