ELISA Tutorial 6: How to Analyze ELISA Data with GraphPad Prism

Here, we demonstrate how to analyze some typical ELISA using software called GraphPad Prism. This software allows us to effortlessly fit a curve to our standards, and then use that curve to interpolate the concentration of our samples.

This video is a part of our ELISA Tutorial Series. Here are all of the videos in this series:

We hope you enjoy watching and benefit from our tutorials. If so, please take a minute to "like," or better yet, share them with others!

Thanks for watching!

Youssef Farhat

***Check out some of our other tutorials via the links below***

[erythreal]

I'm so thankful for your ELISA tutorials! Without them I'd be failing my uni assignments, thank you so much!!!

[Protocolplace-about]

I'm glad the video is helping!  Best of luck with your studies : )

[anata]

Your video presentation is very clear, easy to understand. Very helpful, especially on how to deal with the data. Thanks a lot.

[rimapant]

Explanation is very clear and simple. This tutorial series is very helpful.
Thanks for the all the effort and time you putting into it.
Waiting to see more. 

[Smilesss]

Wow. This was the best tutorial of anything I've ever seen before.

[zeelbhatia]

thank you for this video. you made ELISA assay easy.

[sadehavvad]

Thank you! I am very happy that I made it all by myself :)

[royzhang]

thanks so much for this tutorail and your selfless dedication

[carlitosalex]

This video was sensational, very clear and objective. Even with a previous version of the GraphPad Prism, the explanation was very helpful. Thanks a lot !!!

[nurainmfuseini]

youssef! Glad to see you are doing great things! hope all is well!

[sheilaspadaphd]

Hi, many thanks for your help! quick question, is the blank subtracted? Thanks!

[missroua]

thanq so much, still waiting for the sequel :)

[mustanghmt]

The tutorial is very helpful, could you please explain (the next step) how to use the obtained concentration and the other data to graph the quantified protien  and show the error bars

[carinebokop]

Hello, thank you so much for the videos, Is there a following tutorial ? tutorial 7? Or are there other videos on Gaphpad prism beside tutorial 5 and 6?

[yvgnoek]

That was really helpful. Thank you so much! The problem I am experiencing is that Prism would just "make up" values for the readings that were negative or 0. Even though in one run I had a zero standard given with the absorbance and concentration of zero it would calculate a concentration of 50 for another sample that had the same absorbance?! Any suggestions on how I can prevent this, so that my interpolation will also just not give me any result for the samples at or below 0?

[medicalresearchcentre-kast]

Thank you for the tutorial. I use graph pad Prism 8. Please help me as my Table of Results is missing so I cannot determine R2

[peterfarsta]

Thanks alot. This video helped me heaps!

[hassertoo]

Can someone tell me if it isn't the opción of interpolate standard curve in graph pad prims how can I do this analysis? Thanks

[Unpopular-channel]

thank you, exellent .can you do pcr the same way please.

[gengpan]

thumb up. if the curve is not good one what should we do then?