Genetic Engineering of Cells Based on Selection and Growth on Bacteria | Protocol Preview

Genetic Engineering of Dictyostelium discoideum Cells Based on Selection and Growth on Bacteria - a 2 minute Preview of the Experimental Protocol

Peggy Paschke, David A. Knecht, Thomas D. Williams, Peter A. Thomason, Robert H. Insall, Jonathan R. Chubb, Robert R. Kay, Douwe M. Veltman
MRC Laboratory of Molecular Biology,; University of Connecticut, Department of Molecular and Cell Biology; Cancer Research UK Beatson Institute Glasgow,; University College London, MRC Laboratory for Molecular Cell Biology; University College London, Department of Cell and Developmental Biology;

Dictyostelium discoideum is a popular model organism to study complex cellular processes such as cell migration, endocytosis, and development. The utility of the organism is dependent on the feasibility of genetic manipulation. Here, we present methods to transfect Dictyostelium discoideum cells that overcome existing limitations of culturing cells in liquid media.

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