Time Lapse Imaging of Macrophage Chemotaxis | Protocol Preview

Time-lapse Imaging of Mouse Macrophage Chemotaxis - a 2 minute Preview of the Experimental Protocol

Esther van den Bos, Stefan Walbaum, Markus Horsthemke, Anne C. Bachg, Peter J. Hanley
Institut für Molekulare Zellbiologie,;

Here we describe methods using time-lapse, phase-contrast microscopy to image mouse resident peritoneal macrophages in a chemotactic complement C5a gradient. The protocols can be extended to other immune cells.

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