Understanding Bioinformatics File Formats: GFF3 and GTF

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This is a quick video going over the specifics of GFF3 and GTF files. In this video, I will go over various fields in the GFF3/GTF files while taking a look at an example GFF3 and GTF file, understanding how the data is organized and where these files are used.
I hope you find this video helpful! Leave your thoughts in the comment section below!

Chapters:
0:00 Intro
0:33 Structure of a eukaryotic gene
1:40 What type of data is stored in these files? (GFF3 and GTF)
2:19 How is the data organized?
2:47 Looking at an actual GFF3 file
4:01 Going over 9 columns in GFF3 file
6:37 Understanding how data is hierarchically organized in GFF3 file with an example
9:45 Looking at an actual GTF file
11:01 Where are GFF3/GTF files used?

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Starting with very basic concept and then taking it to the next level --- wow -- Beautifully explained.

aamirmalik
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Explained very clearly. Easy to understand

sruthisrinivasan
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Since you are discussing file formats please discuss VCF and BED formats too. It would be a great help.

stemcell
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Thank you!! At 7:06 where is the upper part of the slide taken from?

wangnan
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You the best! How can some use RNA seq data to validate if annotations are correct?

lukanyomakhabane
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hii. thanks for making such beautiful videos. A little request. Can you please make a video on analysing rna-seq data on the usegalaxy web tool? thanks.

S_M_LA
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Hi thanks for your videos.
Why do u not start a course with very basic coding detail . As I dont have experience in coding and find it difficult to understand the scripts language.
Moreover I would appreciate your if u start a series of videos with aim.of teaching analysis of GWAS studies and applying statistical models on data.

MZMcrypto
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Hello. Thank you very much for the video.
Can you make a video on GWAS.

Thank you

abhisheknayak
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Hi . Can u make tutorials for Hi C and HiChIP data analysis

priyankabiotech