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Understanding SAM/BAM file specifications
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The sequencing Alignment Mapping (SAM) file was designed to store the mapping data of a sample against a reference genome in a way that allow for easy downstream analysis.
In this video, I hope to go through the basic SAM file specifications based on the original documentation from Samtools. SAM file mostly consists of two sections, a header section that records information on the reference genome and experiential details, and an alignment section that records the individual nucleotide sequence difference between the samples and the reference.
Link to SAM specifications
Link to Slides
Link to sample SAM file
How to read Cigar String
In this video, I hope to go through the basic SAM file specifications based on the original documentation from Samtools. SAM file mostly consists of two sections, a header section that records information on the reference genome and experiential details, and an alignment section that records the individual nucleotide sequence difference between the samples and the reference.
Link to SAM specifications
Link to Slides
Link to sample SAM file
How to read Cigar String
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