Luciferase reporter assay | What is luciferase assay used for? | Applications of Luciferase assay

This video talks about the Luciferase reporter assay. We will learn what is luciferase assay used for and finally, we will look at the applications of the Luciferase assay.

Also follow me on other social media -

For Physics part of bio exams - Physics for IIT JAM biotechnology

#Luciferasereporterassay #Whatisluciferaseassayusedfor #ApplicationsofLuciferaseassay
#animated_biology #animated_biology_with_arpan #biology #bio_facts #CSIR_NET #IIT_JAM #IIT_JAM_BT #biotechnology #bioscience #biological_sciences #microbiology #genetics #zoology #molecular_biology #cell_biology #botany #immunology #ecology

0:00 Luciferase reporter assay
1:31 Promoter is cloned upstream of luciferase sequence
3:56 How to understand whether the promoter is strong or weak?
6:44 A normalized ratio is obtained for each well by calculating
7:21 The fold change in activity between two test groups using the following equation

[jonashoffmann]

The best Video I've found. Thanks man❤️👍

[maziastro]

5:47

How would we know whether the promotor is NOT BEING EXPRESSED AT ALL or is a REPRESSOR?

[williamcheng]

the GOAT appears when we need him most

[JaceTan-]

Clear, visually simple to digest and easy to recall! Many thanks.

[muditgupta]

Hi, Thanks Arpan, wonderfully explained!!!

[percypercy]

Thank you so much. This video was extremely helpful

[jaimayy]

I'm writing an essay on luciferins in biomedical research, and this video has been incredibly useful! thank you so much!!!

[mamacocolilo]

So much about this bothers me. Starting with the first 7 letters of this chemical's name. Good presentation though. Thank u

[guihuajia]

Hello, could you please let me know how to produce dsRNA?

[jayashiTGUY]

If we are expressing our TF of interest, then we should also have to normalise it via western blot, isnt it?

[DrJacob-zl]

Why it’s in a vector ?
Why they trying to mess with human genomes ?

[bizhenyu]

This explanation is really good, thank you!n

[julia-hoyg]

omg this explanation was so good, thank you!

[juthibiswas]

Nice explanation. Could you please make videos on TF profiling?

[星胡-rq]

hello thanks for shareing this video to explain the assay!my question is where renilla internal control gene insert to the gene of interested?tyvm

[InnovativeSaint]

I know Lucifer means light, and it would seem many advances in medicine have a Latin predisposition, nevertheless, Lucifer race (how it sounds) seems quite interesting.

[AmruMagdy]

images (2) ببب«О Боже, позволь нам достичь Рамадана, достижения, которое изменит нашу ситуацию к лучшему, очистит наши души и наши внутренности, достижения милосердия, прощения и свободы от ада».