Live Imaging of Viral Infection Using Luciferase Fluorescent Reporter

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection - Experimental Protocol

Influenza A viruses (IAVs) are contagious respiratory pathogens that cause annual epidemics and occasional pandemics. Here, we describe a protocol to track viral infections in vivo using a novel recombinant luciferase and fluorescence-expressing bi-reporter IAV (BIRFLU). This approach provides researchers with an excellent tool to study IAV in vivo.

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