Converting BAM to BigWig

Next we will convert the extracted reads in their BAM format to the BigWig format.
The BigWig format is for display of dense continuous data that will be displayed as a graph.
In our case this would be the gene expression pileup denoted by the gene expression value FPKM, or fragments per kilo base of transcript per million mapped reads.
We will start by converting the knockout read data.
Select the pencil icon.
Under the "Attributes" tab, assign the mouse reference genome mm9 from NCBI.
Then, select "Save".
Select the "Convert Format" tab.
Then "Convert BAM to BigWig".
Select Convert.
Note: these steps will also have to be performed on the wild-type read data.