How does a spectrophotometer work?

Here's how a spectrophotometer works. A lamp provides the source of light. The beam of light strikes the diffraction grating, which works like a prism and separates the light into its component wavelengths. The grating is rotated so that only a specific wavelength of light reaches the exit slit. Then the light interacts with the sample. From this point, the detector measures the transmittance and absorbance of the sample. Transmittance refers to the amount of light that passes completely through the sample and strikes the detector. Absorbance is a measurement of light that is absorbed by the sample. The detector senses the light being transmitted through the sample and converts this information into a digital display.

[hani]

This video is 100× better than a 15 minute video trying to explain the same concept.

[giggogalac]

I believe that in the future, videos like these should 'do the talking' in classrooms, especially when the topic is complicated. The professor explains very well but in this case could not be better than a simple, yet effective video. *(P.S. this video summarized my professor's three-month explanation.)*

[Duckarefun]

So I'm just here doing some chemistry work, this is like the first and shortest and most informative video with a mega helpful link. love you guys

[fireflysci]

Wow this was a sweet and informative video. Thanks so much for making it. It made it crystal clear how a spectrophotometer works.

[spikeoac]

This is so awesome. I love it when educational videos are concise.

[friedrichbaumgarten]

Brilliant! Crystal clear and straightforward. It helped a lot; Thanks!

[gillibean]

Great short and simple explanation. Thanks!

[traskisgi]

thank you, really simple, quick and easy to memorise info!!

[moustafasaadallah]

I believe that in the future, videos like these should 'do the talking' in classrooms, especially when the topic is complicated. The professor explains very well but in this case could not be better than a simple, yet effective video.
very good video

[jozitro]

This patent is like a forgotten principality that measures the spectrum of ignition refraction of when all things were one and to return back in time where all things have become separated.

[OliverG]

I wonder if you could combine this with computed tomography to get slices of absorptions in pigments?

[rogernevez]

Do you think it is possible to use a similar equipment to identify the element composition of a metal surface?

[ramiskhan]

how can absorbance be used to determine which solution (if you are testing many solutions) can be used to determine which has the darker or lighter shade of that particular colour?

[abdallahsabry]

How simple and smart device, thanks sir.

[mehtababbas]

This video made spectrophotometry concept so clear 😙😍😘

[Jaberising]

Thanks you've solved my homework ;)

[cassidyhill]

Does anyone know what is causing the diffraction grating to rotate though, like is it the wavelength being adjusted?

[username]

What is measuring the absorbance of the light by the sample worth for? What important information does that give?

[yuval]

thanks! finally something understanable

[fatimaanjum]

I was studying for hours about spectrophotometer.. But this One minute is better than those 👌🏼