W16: Library Prep for NGS- Day 2

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This workshop will cover the basis of Next-Gen Sequencing Library Preparation for Illumina Sequencers. Different Library Preparation Techniques (DNA-seq, ChIP-seq, RNA-seq, Methyl-seq) are explained the first and second day in class. The third day, Quality Control steps of the starting input material and final libraries are performed in the Lab (TapeStation). Purification from Primer Dimers (using AMPure beads) and Library Submission is also performed on the same day.
  1. UCLA QCBio Collaboratory
  2. Library Prep
  3. NGS
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Автор

Hi! thank you for you videos, im not in your program, but your lectures were the most useful in the internet. wish all the best in your work!

ylidene
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31:00 chip/atac-seq start from chromatin. protein contamination is a norm.

jkim
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1:08:00 intact RNA vs fragmented RNA. e.g. FFPE

jkim