Western Blotting Webinar: the principles and a comparison of indirect vs. direct immunodetection

Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. Although Western blotting is simple in principle, for a successful outcome, scientists must be aware of the technical caveats which must be overcome. Therefore, to provide further insights in to Western blotting, this exciting webinar discusses:

• Sample preparation
• SDS-PAGE
• Western Blot transfer
• Blocking
• Indirect Detection -- primary antibody incubation and indirect detection
• Indirect Vs. Direct antibody detection -- advantages and disadvantages
• Direct detection for multiplex fluorescent Western blotting
• Problems with sourcing labeled antibodies for direct detection
• The solution simple antibody labeling kits
• Questioning the secondary antibody amplification hypothesis

This video was produced by Innova Biosciences which is now fully integrated with Expedeon Ltd.