2.15 - Knife Sterilization without Fire Hazard

⚙️➡️🍄 In this video I teach you how to sterilize your knife with an only chemical ethanol sterilization. The benefit is, that you reduce the fire hazard and the blade does not get blunt. One need to sterilize the knife before performing agar mycelium work with your petri dishes when you want to clone your mycelium.
There are two tubes involved. One is closeable for storage and the second one is open for immediate usage during the process.

🍄 Here you will find the .pdf script for printing and offline use!

🛠️ Here you will find the .stl files for 3D printing!

🕘✅🍄 Jump marks:
◼ Introduction: 0:00
◼ Support: 1:08
◼ Specifications: 1:25
◼ Ethanol disinfection: 1:41
◼ Blunt Blade vs Sharp: 8:41
◼ Bill of Materials: 10:26
◼ Closure: 11:48

#mushroom #sterilization #knife #ethanol #petridish #mycelium #cloning #agarwork #laboratory #mushroomcultivation #diy #homesteading #sustainability #healthylifestyle

[linusgoblin]

Hey, heres my anti-thesis,

It sure looks safer, but to me the many disadvantages ( time needed between transfer, wiping the blade, having alcohol that will transfer to the agar plates, making a aseptic alcool soup, not having certainty about the sterilization of the blade) weight out against the only advantage which is maintaining sharpness of the blade.

And is that whole process really saving money? Scalpel blades are 10ct€ piece in a 100pack, how much alcohol does this consume to save one blade?

If one just want to avoid fire hazard i recommend making a DIY induction induction coil sterilizer.
This tek goes against all i have learnt (alcool sanitize, heat sterilize).

Cheers

[MiguelRodriguez-wnmc]

Hi, good video and very interesting explanation. I have a question about the material you use to prototype the piece. What material do you use to do 3D printing? (Sorry if you mention it at some point in the video, it's because when the video is automatically translated, that information doesn't appear.) I read an article some time ago that certain prototyping materials are more likely to contain microorganisms than others due to their composition, roughness, etc. Well, great video.