What are PYR1 biosensors?

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This is an overview video of the function and uses of the PYR1 biosensor platform. PYR1 functions by binding to a ligand and then binding to its dimer partner, HAB1. This causes downstream cascades in the cell or in an in vitro assay. The PYR1 biosensor has been demonstrated as an in vitro tool as well as an in vivo tool in plants, yeast, and bacteria.
This topic was about half of my PhD! If you have any questions, leave them in the comments!
Here is some further reading:
Titled "Rapid biosensor development using plant hormone receptors as reprogrammable scaffolds"
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[DIYBiotech]

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[madiparks]

This is super exciting research!!! I am a junior majoring in bioengineering and I hope one day this technology wide-spread, amazing!

[That_Chemist]

How do you control for false positives for environmental testing of pesticides using PYR1? are they that ligand-specific?

[devilsolution]

Very interesting, testing for fent or zenes on the fly would be great

[itspranavs]

Great video my guy. You got yourself a new subscriber. I have a question, This method is a simple yes or no right. It's not a quantitative test ?

[devilsolution]

Talking about biotech specifically id like to know more about BDNF and peptides personally

[warlokyx]

Org. Chemist here, and I wonder what's the false negative rate on these sensors. Just from a combinatorics pov if we assume that it's mostly the binding residues that determine the binding to the ligand, that leaves us with somewhere around 20^10 options. That's still approx. 10^13 which is just over the number of small molecules accounted for in the chemical space project. So even undercounting the variability space of the sensors, it is bigger than the variability space of the signal that they are sensing (ligands) meaning one would expect just by chance that the same ligand would be sensed by multiple sensors, leading the false negatives. This is ofc a statistics argument for why false negs would be expected but how is this solved from a bioengineer's pov?

[Daan]

Hey man can you review this really interesting paper on frontiers. It's called

Construction of an efficient Claviceps paspali cell factory for lysergic acid production.

Would be amazing if you would make video of that. Really interesting study you did. And congratulations with your getting your diploma.

I like your video styles it's really good education and you explain complex topics in an easy way.

[beckettsebolt]

Are there limits of detection for PYR1 biosensors? Is the downstream signal cascade dependent on ligand binding affinity? I guess I'm wondering if these biosensors are only capable of spitting out a binary yes or no response to the presence of some ligand. Or is there a way to somehow account for the degree to which GFP is expressed and then do a back of the envelope calculation for analyte concentration?

[synchro-dentally]

Could make videos on the manufacturing of Ivermectin and Calcifediol?

[jaylingreen]

Thank you for your and your peers work. The world needs more greatness like this. Also lemme know when you get some of that special yeast🫡🤫

[Acceleratedpayloads]

You should do a book review of blood music by greg bear