Ribosome Protein Synthesis Study by Single Molecule FRET | Protocol Preview

Single Molecule Fluorescence Energy Transfer Study of Ribosome Protein Synthesis - a 2 minute Preview of the Experimental Protocol

Yuhong Wang
University of Houston, Department of Biology and Biochemistry;

Single molecule fluorescence energy transfer is a method that tracks the tRNA dynamics during ribosomal protein synthesis. By tracking individual ribosomes, inhomogeneous populations are identified, which shed light on mechanisms. This method can be used to track biological conformational changes in general to reveal dynamic-function relationships in many other complexed biosystems. Single molecule methods can observe non-rate limiting steps and low-populated key intermediates, which are not accessible by conventional ensemble methods due to the average effect.

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