How to prepare plant extracts using various solvents||Methanolic extract

Learn how to prepare various plant extracts using different solvents for biochemical assays, HPLC, GC-MS studies etc.
Do watch the biochemical assays in my other videos to know how to analyze the proteins, carbohydrates, proline, phenolics, flavonoids and many more...

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Thank you!

[zs]

I am following your videos in my PhD work

[ABCDEFGHIJKLMNOPQ]

That was so informative thank you so much. I was struggling with this to understand through paper for so long and your video cleared everything

[zs]

Your video is extremely informative Sir. Thank you so much 🙏

[rohitpaithane]

Hello Sir. I have some queries about plant collection and drying processes.

1. Can we pluck leaves and leave the plucked leaves to dried separately?
2. Can we cut the roots and leaves separately and dried and powdered at the same time.
3. How long time we should dry the plant sample in shaded area?
4. After powder prepared, how long time we can use powder to carry various experiments?

I request you to please answer my doubts.

[zs]

Please keep posting more videos regarding phytochemical analysis of the plant and also about essential oils of the plants.

[satyamt.]

Thank you so much. Can you share plant extract preparation in different solvent for GCMS analysis, so that I can send the sample for analysis to other lab. Thanks again.

[nusratbibi]

Sir very good job done by you kindly share videos related to bacterial research related to metal NPs and ethyl acetate extraction of bacterial metabolites

[ankitachowdhury]

Thnkyou so much sir for your effort....your videos are very beneficial

[bintenaeem]

sir, My topic of research is to determine the invitro antidiabetic properties of the medicinal plants. A humble request to you to make a video on this assay.I always watch your videos before performing assays.plz make the video as soon as possible .

[muntasirkamal]

Thank you for the clear explanation. I have a question- after methanol extraction and evaporating the methanol, when you re-dissolve the plant residue, can you use any solvent other than methanol? Can I re-dissolve the residue in DMSO to make 1 mg/mL extract in DMSO? Will it dissolve in DMSO? For my experiment I cannot use methanol. It must be DMSO that's why I am asking. Thanks.

[aminaqureshi]

Please make a video on work mechanisms of rotary evaporator? And is there any simple method to remove solvent?

[almasmulla]

Very informative video sir. Thank you for uploading. I have a plant whose phytoconstituents are not known. M planning for tlc bioautography can you help me how to select solvent system?

[MHui-njdq]

Thank you for the video, sir. I was wondering what concentration of extract (in mg/mL) is needed for the DPPH assay? Thanks!

[sakshithakur]

Hi sir
Just need to know one thing, I have done serial plant extraction using pet ether, chcl3, and ethanol.. so will u plz tell me how can I make 1mg/ml solution of chcl3 and pet ether extract for TFC TAc, etc. Like which solvent I can use to dissolve these solvent extract for my further analysis

[deannaaudrey]

Thank you for the video! My project is to identify the best brewing temperature and time for green tea leaves to exhibit the most antioxidant capacity. Can I directly use the sample ( solution of tea and water) to carry out DPPH essay?

[varshinipreethi]

Sir wonderful explanation i have a doubt which analysis is best to test the phytochemicals in plant extract? Is GCMS is good?

[tejashreesande]

very very useful video...sir will you please tell..for extraction how many solvents we can take ideally?

[priyanshukul]

Are these extracts can be consumed by normal person?

[kalpanarautela]

Thank you for easy explanation of procedures.
Sir, could you please provide a reference for this procedure... ?
Thank you

[mehwishhameed]

If we do centrigation, then how we can make different concentration of extract for antibacterial activity?