How to Stain an SDS-PAGE gel

Your gel just finished running -- what do you do next?

In this video, Dan shows you how to disassemble your SDS-PAGE gel, and how to stain it using either PageBlue, Coomassie, or Silver Staining.

Coomassie is the older method of staining, and gives bright blue bands on the gel. PageBlue is similar, but provides a quicker alternative. If you have faint bands and require a more sensitive method, you can use Silver Staining.

Choose whatever method you need, but beware of the hazardous materials that may be used.

[Findulidas]

Very useful. My teacher/lab supervisor had us set up a whole sds-page lab from scratch complete with method, volymes and what we do how in what order. We had to find information ourselves on how to do it using books and online. Most of us had not seen this been done before. These clips really helped me to visualize what Im supposed to do which is something modern teaching has forgot the imporance of.

[WinterButterfly]

Thanks for posting these videos. You've been very helpful!

[TheDKDEO]

can you able to provide detail protocol how i can run a page gel and try to do stain. please help me to learn

[RekaRoams]

Great video!
What should have been said for silver staining is that band intensities CANNOT be directly compared in silver staining.

I forgot the precise reasoning, but certain amino acid rich proteins are more likely to bind with the stain, hence, bands cannot be compared.

[labtricks]

@osmarguz you can buy it directly from the manufacturer, Fermentas, which is now a part of Thermo Fisher. Try looking at their online catalogue for the prices

[dariacirlan]

was looking at this out of curiosity for my MBB222 class at SFU when I realized where this was filmed haha. Great video though!!!

[kylehasgeniusbits]

I have a question, what about the next step of preserving your gel in drying solution? What's the best way to do that?

[rhodnius]

Hi, thanks for sharing. I have a question. What's the reason for microwaving the gel? Thanks :)

[carlypena시리우스]

So useful! But i would like to see the final result comparing the three methods.

[laurenmabe]

OK, so I tried to stain my membranes and got really poor results. My standard bands are clearly visible and did absorb the Coomassie blue, but no sample bands were stained. Was this because I stained the membrane as opposed to the gel itself, or will it also work on the membrane? Thanks!

[nidhivijayan]

What does the gel look like in the end??

[coolkolu]

we generally use Coomasie Blue fr staining..

[andreialourenco]

Yes. This video is great.
But i would like to know what is making my buffer get too much hot while my SDS-Page gel is running. And if it can damage the proteins. Thank you.

[p.m.satyanarayana]

I saw my gel getting shrunk after staining & destaining (CBB). Staining- 10 mins- generally I stain it for 10 mins & destaining-30 mins.

[teresastories]

I’m the guy on the door who is softly drinking his coffee

[komals.]

Hii make a vedio on native gel electrophoresis too

[CherryMoch]

can you make one for western blotting? thanks! :)

[labtricks]

Distilled water should be fine to separate the gel, and then follow your procedure as usual for Western blot. Just remember to use clean gloves when handling this gel, the membrane, and filter papers during the process.

[DanzQueen]

Oooh yeah, Page Blue is new. Never used it. Must try...

[lmtrevino]

what happens when you don't add SDS