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2D Poly Acrylamide Gel Electrophoresis
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Two-dimensional Gel electrophoresis was first introduced by P. H. O'Farrell and J. Klose in 1975. Two-dimensional Gel electrophoresis is a powerful and widely used method for the analysis of complex protein mixtures extracted from cells, tissues, or other biological samples. This technique sorts proteins according to two independent properties in two discrete steps: the first-dimension step, isoelectric focusing (IEF), separates proteins according to their isoelectric points (pI); the second-dimension step, SDS-polyacrylamide gel electrophoresis (SDS-PAGE), separates proteins according to their molecular weights. Applications of 2-D Gel electrophoresis include proteome analysis, cell differentiation, detection of disease markers, monitoring therapies, drug discovery, cancer research, purity checks, and microscale protein purification.
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