De novo peptide sequencing from mass spectrometry data

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Matt Padula

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You're a legend! This is currently helping me with my mass spec lab in my biochemistry degree 💪🏽📊

TJ-dowt

Super great! Thank you very much. It is very helpful to me, a beginner of MS user

jingjingxu

This saved my skin, thank you so much! Succinct and easy to understand.

mamarain

This video was really a life-saver. Thank you!!!

niloofarfam

This Video is extremely helpful, thank you so so much!

xuejie

Thank you for your kind explanation :) This is very helpful

둥글-sy

Just the information i needed! Thank you sir :)

TheMistakentruth

Thanks a lot for these videos. However, a doubt remains.
The peptic fragments are just being blasted with inert gas to break them down into b and y ions.
Where from are the extra 1 or rather 2 H+ at the N terminal of 'y-ions' coming from, giving them overall +1 charge?

acell

This was very helpful but I still have one question. I don't get why the relative intensities of complementary b and y fragments are different, like b2 and y10 in your example. I mean, when the parent ion fragments on a specific bond you get the same amount of the two complementary fragments, so I really don't get this. could you help me please?

lucab

when digest with trypsin, it cuts at the c terminal side of Arg and Lys, so the Arg and Lys should be at the terminus of b ion, isn't it. I don't get how is it at the y ion? Could you please explain

doudna

Hey sir! Thanks for the help- question I had is how would you draw a simple mechanism for the y and b fragmentation? How do both the nitrogen np oxygen become positively charged? Thanks for the vid!

AnniePrettyFace

this is really informative video, thank you so much, can we have the ppt version i want to study it as well

axalnas

I have a questions I did all this, b ions I got sequence of RYAAYKYA
and for y ions I have the sequence of IKAYKYAA
I am not sure what's my final sequence going to be

FunTime-hdzz

De novo peptide sequencing from mass spectrometry data

De novo peptide sequencing from mass spectrometry data

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