Determining 3D structures of macromolecular complexes using Cryo-EM to understand function

"Holger Stark from the Max Planck Institute in Göttingen, Germany discusses current advancements in cryo-TEM to examine the 3D macromolecular complexes that reveal new functional processes and mechanisms critical for the fundamental understanding of life.

Cryo transmission electron microscopy (cryo-TEM) is increasingly becoming a mainstream technology for studying the architecture of cells, viruses and protein assemblies at near atomic resolution to reveal new functional processes and molecular interactions. Cryo-TEM is a powerful technique that can provide the three dimensional structures of large protein complexes that are more challenging to determine by other biophysical techniques including X-ray crystallography and NMR spectroscopy. In addition, the combination of cryo-TEM with X-ray crystallography and NMR spectroscopy results in a more comprehensive understanding of the structure-function relationships of macromolecular complexes than could possibly be achieved with any single technique alone.

Providing an automated workflow from sample preparation, to image acquisition and reconstruction, to final visualization - Thermo Scientific Structural Biology solutions enable structural biologists to gain a greater insight into the architecture and shape of macromolecular complexes and their functions."

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